Genetic diversity analysis and DNA fingerprinting of tomato breeding lines using SSR markers
Al-Shammari, Aziz Mahdi Abd
Hamdi, Ghassan Jaafar
Al-Mahdawi, Muthanna Abdulkhader Salh
Mohammed, Noora Khalifa
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There is a need to expand the information on genetic relationships between tomato (Solanum lycopersicum L.) lines to improve hybridization breeding. The genetic diversity and relationships among 24 tomato lines were evaluated by simple sequence repeat (SSR) markers. A total of 65 bands were generated with 15 SSR primers, of which 64 bands were polymorphic. The mean polymorphic information content was 0.356. There was a high degree of polymorphism between tomato cultivars. The mean marker index and heterozygosity were 0.045 and 0.454, respectively. Cluster analysis grouped cultivars into 6 main clusters. The cvs. Mo. H. P, 'C. C. Orange', and 'Marb' had the greatest genetic distance from other cultivars and is suitable for hybridization to achieve maximum variability for selection in segregating populations. The data can be used to select appropriate parents in tomato hybridization breeding.