VLI projektid

Selle valdkonna püsiv URIhttp://hdl.handle.net/10492/8657

Sirvi

Nüüd näidatakse 1 - 20 40

Antibacterial and antibiofilm potential of potato peel derived extracellular vesicles against Bacillus cereus ATCC 11778: A proteomic perspective : [poster]
(Estonian University of Life Sciences, 2024) Ekanayake, Gayandi; Sapugahawatte, Dulmini Nanayakkara; Godakumara, Kasun; Midekessa, Getnet; Roasto, Mati; Bhat, Rajeev; Fazeli, Alireza
INTRODUCTION • Plant derived extracellular vesicles show great promise as antimicrobial agents due to their diverse cargos. • Bacillus cereus , a gram positive pathogen, causes infections and food spoilage, making its control crucial for food safety and public health. • Over a series of in vitro microbial assays Potato peel derived EVs (PPEV) have shown potential in controlling growth dynamics of B. cereus. • The aim is to understand the EV proteome’s role in the antimicrobial activity of PPEVs and elucidate their mechanism of action in silico.
Antibiotic Resistance in Campylobacter spp. Isolated from Broiler Chicken Meat and Human Patients in Estonia
(MDPI, 2022) Tedersoo, Triin; Roasto, Mati; Mäesaar, Mihkel; Häkkinen, Liidia; Kisand, Veljo; Ivanova, Marina; Valli, Marikki Heidi; Meremäe, Kadrin
Poultry meat is considered the most important source of Campylobacter spp. Because of ris- ing antimicrobial resistance in Campylobacter spp., this study investigated the antimicrobial resistance of Campylobacter isolates from fresh broiler chicken meat originating from the Baltic countries sold in Estonian retail settings. Additionally, human clinical isolates obtained from patients with Campylobacter enteritis in Estonia were analysed. The aim of this study was to investigate the susceptibility of Campylobacter spp. to nalidixic acid, ciprofloxacin, tetracycline, streptomycin, erythromycin and gen- tamicin. The broth microdilution method with the EUCAMP2 panel was used for MIC determination and antimicrobial mechanisms were analysed using WGS data. A total of 46 Campylobacter strains were analysed, of which 26 (42.6%) originated from Lithuanian, 16 (26.2%) from Latvian, and 4 (6.6%) from Estonian fresh broiler chicken meat. In addition, 15 (24.6%) Campylobacter strains of patients with campylobacteriosis were tested. The antimicrobial resistance patterns of Campylobacter spp. isolated from fresh broiler chicken meat samples of Estonian, Latvian and Lithuanian origin collected in Estonian retail, and from patients with Campylobacter enteric infections, were determined. A total of 46 (75%) of the isolates tested were C. jejuni and 15 (25%) were C. coli. Campylobacter resistance was highest to nalidixic acid (90.2% of strains) and ciprofloxacin (90.2%), followed by tetracycline (57.4%), streptomycin (42.6%) and erythromycin (6.6%). All strains were sensitive to gentamicin. Additionally, antimicrobial resistance genes and point mutations were detected in 27 C. jejuni and 8 C. coli isolates previously assigned as resistant with the phenotypic method. A high antibiotic resistance of Campylobacter spp. in Lithuanian- and Latvian-origin broiler chicken meat and Estonian clinical isolates was found. Similar antibiotic resistance patterns were found for broiler chicken meat and human Campylobacter isolates.
Antimicrobial Resistance of Campylobacter coli Isolated from Caecal Samples of Fattening Pigs at Slaughter
(MDPI, 2023) Tedresoo, Triin; Roasto, Mati; Mäesaar, Mihkel; Fredriksson-Ahomaa, Maria; Meremäe, Kadrin
Pigs are known as the main Campylobacter coli reservoirs. Campylobacteriosis, the most commonly reported gastrointestinal disease in humans, is mainly caused by the consumption of poultry meat, and little is known about the role of pork. Pigs are often associated with C. coli, including antimicrobial-resistant isolates. Therefore, the entire pork production chain must be considered as an important source of antimicrobial-resistant C. coli. This study aimed to determine the antimicrobial resistance of Campylobacter spp. isolated from caecal samples of fattening pigs at the Estonian slaughterhouse level over a five-year period. The proportion of Campylobacter-positive caecal samples was 52%. All Campylobacter isolates were identified as C. coli. A high proportion of the isolates were resistant to most of the studied antimicrobials. The resistance to streptomycin, tetracycline, ciprofloxacin and nalidixic acid was 74.8%, 54.4%, 34.4% and 31.9%, respectively. In addition, a high proportion (15.1%) of the isolates were multidrug-resistant and, in total, 93.3% were resistant to at least one antimicrobial.
Assessment of microbiological safety and quality of plant-based alternative products : [presentation]
(Estonian University of Life Sciences, 2025) Mandel, Tiina; Roasto, Mati; Mäesaar, Mihkel; Meremäe, Kadrin; Sapugahawatte, Dulmini; Reinik, Mari
ACTUALITY OF THE TOPIC • The shift from animal-based proteins to plant-based alternatives has significantly increased in recent years, largely driven by a combination of environmental, health, and animal welfare concerns of consumers. • The market of plant-based alternatives is rapidly expanding and evolving, it is expected to reach around 46 billion euros by 2027. • New technologies and processing methods mostly used for the production of alternative products may pose new food safety risks including microbiological hazards.
Covid-19 mõju mikrobioloogilisele toiduohutusele Euroopa Liidus ja Eestis
(Eesti Maaülikool, 2022) Roasto, Mati; Laikoja, Katrin; Mäesaar, Mihkel
Käesolev artikkel annab ülevaate zoonoossete toidupatogeenide toidus levimuse ning toidutekkeliste haigusjuhtumite suundumustest Euroopa Liidus (EL) k.a. Covid-19 pandeemia võimalikust mõjust toidutekkeliste mikrobioloogiliste haigusjuhtumite esinemisele. Zoonoos on otseselt või kaudselt loomulikul teel looma ja inimese vahel edasikanduv haigus või nakkus (PTA, 2022). Toidutekkelised haigused on infektsioonid või intoksikatsioonid, mis on põhjustatud saastunud toidu või vee kaudu organismi sattunud bakteritest, viirustest, parasiitidest või keemilistest ainetest. Toidu saastumine võib aset leida toidu (esma)tootmise, töötlemise ja tarbimisahela mis tahes etapis ning tuleneda keskkonnasaastest, sealhulgas vee, pinnase või õhu saastumisest, aga ka toidu mitte nõuetekoha- sest ladustamisest, töötlemisest või muul viisil käitlemisest (Roasto, 2019). Salmonellad, kampülobakterid ja enterohemorraagiline Escherichia coli on kõige levinumad toidupatogeenid, põhjustades igal aastal miljonitele inimestele kerge või raske kuluga haigusi, enamasti soolenakkusi, kuid mõnikord võivad need haigused põhjustada ka inimeste surma (WHO, 2022). Kõige sagedamini esinev zoonoos EL-is on juba alates 2005. aastast kampülobakterioos (Whitworth, 2021). Eestis registreeriti inimestel kam- pülobakterioosi rohkem kui salmonelloosi esimest korda 2013. aastal. Nii on see olnud ka järgnevatel aastatel Eestis.
The effect of pasteurized milk extracellular vesicles on bacterial growth
(Estonian University of Life Sciences, 2023) Sapugahawatte, Dulmini Nanayakkara; Midekessa, Getnet Balcha; Godakumara, Kasun; Prasadini, Madhusha; Kodithuwakku, Suranga; Roasto, Mati; Andronowska, Aneta; Fazeli, Alireza
Extracellular vesicles (EVs) are released by cells and have a lipid bilayer structure. EVs harbor various molecules, including proteins, RNAs, and DNAs. Studies of mammalian EVs are increasingly attracting the interest of researchers; however, there are only few studies of nanoparticles in food. Milk-derived EVs can survive high temperatures and digestion process, while retaining their biological activity. This study investigated the effect of pasteurized cows’ milk derived EVs on growth of five different bacteria. Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 53868, Bacillus subtilis, Bacillus cereus and Pseudomonas aeruginosa with a concentration of 1x107 CFU/ml were separately co-cultured with pasteurized milk derived EVs (1x109 EVs/μl) in Muller Hinton broth. The bacterial growth was monitored as absorbance at 620 nm over 24 hours. Bacteria treated with phosphate buffer solution (PBS) were considered negative control throughout the experiment. The percentage bacterial growth difference was COMBIVET & OH-BOOST JOINT CONFERENCE 2023 55 determined with respect to negative control and results expressed as mean ± standard error of mean. All analyses were performed in three biological triplicates and each biological replicate consisted of three technical replicates. Co-culture of bacteria with milk EVs demonstrated that EVs could decrease the growth of S. aureus, B. subtilis, B. cereus and P. aeruginosa. Highest growth inhibition was observed for B. subtilis (33.9% ± 2.4) followed by B. cereus (18.1% ± 3.7) at 6 hours of incubation. S. aureus and P. aeruginosa growth were inhibited by 12.9% ± 1.3 and 9.9% ± 3.5 respectively after 9 hours incubation. Only the S. aureus growth inhibition at 9 hours was statistically significant (P=0.022) according to T-test. However, E. coli growth was not affected by milk EVs compared to the control. In conclusion, the dietary EVs can be absorbed by bacteria and pasteurized milk derived EVs has a selective inhibitory activity on the growth of some bacteria.
The Effect of Pasteurized Milk Extracellular vesicles on Bacterial Growth : [poster]
(Estonian University of Life Sciences, 2023) Sapugahawatte, Dulmini Nanayakkara; Midekessa, Getnet Balcha; Godakumara, Kasun; Prasadini, Madhusha; Kodithuwakku, Suranga; Roasto, Mati; Andronowska, Aneta; Fazeli, Alireza
Milk-derived extracellular vesicles (mEVs) have gained attention for their potential biological activities, including antibacterial properties and they can carry bioactive molecules. The mEVs can survive high temperatures and digestion processes while retaining their biological activity. Studies of mammalian EVs are increasingly attracting the interest of researchers; however, there are only a few studies of mEVs’ antimicrobial effect.
EMÜ VLI toiduhügieeni ja ohutuse üksuse õppe ja teadustöö : [esitlus]
(Eesti Maaülikool, 2024) Roasto, Mati
Toiduohutuse seminar "EMÜ VLI toiduhügieeni ja –ohutuse üksuse õppe- ja teadustöö, projektide PRG1441 ja TEM-TA52 tutvustus Regionaal- ja Põllumajandusministeeriumi Toiduohutuse osakonnale", 14.11.2024, Eesti Maaülikool, Tartu.
EMÜ VLI toiduhügieeni ja –ohutuse üksuse teadustöö ning koostöövõimalused ettevõtetega : [esitlus]
(Eesti Maaülikool, 2023) Roasto, Mati
Toiduhügieen ja selle eesmärgid: • Toiduhügieen on kõik tingimused ja meetmed, mis on vajalikud toidu ohutuse ja sobivuse tagamiseks toiduahela kõigis etappides – Codex Alimentarius, Food Hygiene, Fourth Edition, Rome 2009. • Toiduhügieeni eesmärk on kaitsta toitu saastumisohu eest e. toidu kaitsmine mikrobioloogilise, füüsikalise ja keemilise saastumise eest – toidu kontaminatsiooni ennetamine või minimaliseerimine. • Takistada mikroobide paljunemist tasemeteni, mis võivad põhjustada tarbijate haigestumist või toidu liiga kiiret riknemist: – hävitada toidus esinevad patogeenid täielikult või tasemeteni, mis ei ületa seadusandluses kehtestatud piirmäärasid. – termolabiilsete toksiinide hävitamine. • Teada on ~200 toidutekkelist haigust.
Fast Protein and Metabolites (Nucleotides and Nucleosides) Liquid Chromatography Technique and Chemical Sensor for the Assessment of Fish and Meat Freshness
(MDPI, 2023) Kuznetsov, Artur; Frorip, Aleksandr; Sünter, Alar; Kasvand, Nensi; Korsakov, Vadim; Konoplev, Georgii; Stepanova, Oksana; Rusalepp, Linda; Anton, Dea; Püssa, Tõnu; Roasto, Mati; Abramova, Liubov; Kozin, Andrey; Toom, Lauri; Hirsch, Soeren; Mukhin, Nikolay
Fast protein and metabolite liquid chromatography (FPLMC) was introduced years ago to enable the easy separation of high-molecular compounds such as proteins from small molecules and the identification of the low-molecular substances. In this paper, the method is applied for the rapid evaluation of freshness and monitoring the aging of animal meat and fish. A novel chromatographic sensor was developed with a deep UV LED-based photometric detection unit (255–265 nm), an original flow cuvette and registration scheme; the processing of a chromatogram with the sensor takes approximately 15 min. Strict isochronism between the elution of ATP metabolites, mainly hypoxanthine (Hx) and inosine monophosphate (IMP), and the time of maturation of meat or fish, was discovered. A new freshness index H* = [Hx]/[IMP] was introduced, which is proportional to the instrumental delay time in the FPMLC chromatograms: the H* index < 0.5 indicates the presence of inosine monophosphate (IMP) and the high quality of the meat or fish. Reasonably strong correlations were revealed between data obtained by FPMLC and total volatile basic nitrogen TVB-N (for fish) or volatile fatty acids VFA (for meat) content. Moreover, putative nucleotide salvage and an increase in the concentration of IMP were observed in fish after heat treatment using the FPMLC sensor and NMR technique.
Food hygiene and –safety research in EMU : [presentation]
(Estonian University of Life Sciences, 2023) Roasto, Mati
Rahvusvahelise projekti INTEREST ("Uuenduslikud tehnoloogiad väärtuslike ühendite saamiseks põllumajandussaaduste ja mereandide tootmise kõrvalproduktidest ja nende toidutoodetes kasutamiseks"; projekti kirje: V230113PKANPA) seminaril prof Mati Roasto suuline ettekanne „Food hygiene and safety research in EMU“.
Food safety of plant based foods : [presentation]
(Estonian University of Life Sciences, 2024) Mandel, Tiina; Mäesaar, Mihkel (advisor); Roasto, Mati (advisor); Reinik, Mari (advisor)
The presentation took place at the EU Food Safety Forum 2024 “Youngs acting for the future of Food Safety in EU”, Rome, December 02-03, 2024.
Growth dynamics of microorganisms in rainbow trout marinated with fruit and berry pomaces : [poster]
(Estonian University of Life Sciences, 2024) Roasto, Mati; Mäesaar, Mihkel; Anton, Dea; Rätsep, Reelika; Elias, Terje; Meremäe, Kadrin
• In recent years, there has been an increasing trend in food production to use various plant-origin materials including fruit, and berry pomaces, a by-products of juice production, in the composition of food of animal origin [1 2]. • This is mainly because of potential health benefits of plant derived bioactive components to human health [3[. • Also, it is in a good agreement with the principles of the circular bioeconomy. The use of berry pomaces reduce the food waste and provides added value to the final products [4].
Growth potential of L. monocytogenes in ready-to-eat minced meat samples enriched with plant powders
(Faculty of Pharmacy, University of Helsinki, 2023) Koskar, Julia; Roasto, Mati; Meremäe, Kadrin; Elias, Terje; Mäesaar, Mihkel; Rätsep, Reelika
Listeriosis is the fifth most frequently recorded human zoonosis in the European Union and one of the most serious foodborne diseases. Regulation (EC) No 2073/2005 lays down the microbiological criteria for Listeria monocytogenes in ready-to-eat (RTE) foods. In case a food business operator wants to apply the numeric criterion of 100 cfu/g to RTE food, a challenge test needs to be performed in accordance with the European Union Reference Laboratory for Listeria monocytogenes (EURL Lm) technical guidance document on challenge tests and durability studies regarding L. monocytogenes. The aim of this research is to provide an overview of the possibilities of inhibiting the growth of L. monocytogenes in minced meat products enriched with plant powders.
Growth potential of Listeria monocytogenesin ready-to-eat minced meat samples enriched with plant powders : [poster]
(Estonian University of Life Sciences, 2023) Koskar, Julia; Meremäe, Kadrin; Elias, Terje; Mäesaar, Mihkel; Rätsep, Reelika; Roasto, Mati
Listeriosis which is one of the most severe foodborne illnesses, ranks as the fifth most commonly reported zoonosis among humans in the European Union To ensure the safety of ready to eat ( foods, Regulation ( No 2073 2005 has established microbiological criteria for Listeria monocytogenes If a food business operator intends to use the numeric criterion of 100 cfu/g for RTE food, a challenge test must be conducted in compliance with the technical guidance document issued by the EU Reference Laboratory for L monocytogenes (EURL Lm Numerous plants have been shown to be effective in inhibiting microbial growth in food The use of plant powders with antimicrobial properties in food manufacturing requires compliance with consumer demands for microbiological safety, nutritional value, and sensory properties The aim of this study was to assess the growth potential of L monocytogenes in minced meat products enriched with plant powders.
Hakklihatoodete riknemise pidurdamine taimsete tootmisjääkidega
(Eesti Maaülikool, 2022) Püssa, Tõnu; Anton, Dea; Rusalepp, Linda; Meremäe, Kadrin; Roasto, Mati; Kaldmäe, Hedi; Bleive, Uko; Rätsep, Reelika
Kuna lihas on palju erinevaid keemilisi ja biokeemilisi (ensümaatilisi) aktiiv- susi, siis on liha, eriti peenestatud kujul, üks kõige kiiremini riknevaid toiduaineid. Teisalt on liha suurepärane kompleks ainetest, mida vajavad kasvuks mitmesugused mikroorganismid, lihal on ka kõrge veesisaldus ja mõõdukas pH. Ensümaatiline autolüüs, oksüdatsioon ja mikroobide kasv on kolm liha riknemise eest vastutavat põhiprotsessi (Iulietto jt, 2016). Mida tugevamini on nimetatud protsessid pidurdatud, seda pikem on liha ja lihatoodete säilimisaeg. Liha riknemine algab juba looma tapmise ajal, kui lihaskoe verevarustus katkeb, seni tasakaalustatud ainevahetusprotsessid blokeeritakse ning lihase enda ensüümide toel algab autolüüs, mis puudutab nii valke, rasvu kui ka glükosiide (Amaral jt, 2018). Jättes autolüüsi eraldi uurimise objektiks, on just oksüdatsiooni ja bakterite kasvu pidurdamine taimsete lisandite abil käesoleva artikli põhiteemadeks. Artiklis käsitletava töö eesmärgiks oli ResTA 14 projekti raames teaduslikult põhjendatud suurima liha riknemise põhiprotsesside pidurdamise võimega bioaktiivsete lisandite väljaselgitamine taimse tootmise jääkides viimaste võimalikult täielikuks ning eesmärgipäraseks ärakasutamiseks (Rätsep jt, 2021). Paljudel juhtudel on taimse tootmise jäägid hoopis rikkamad bioak- tiivsete ainete poolest kui põhitooted, näiteks mahlad (Fierascu jt, 2020).
Hakklihatoodete riknemise pidurdamine taimsete tootmisjääkidega : [esitlus]
(Eesti Maaülikool, 2022) Püssa, Tõnu; Anton, Dea; Rusalepp, Linda; Meremäe, Kadrin; Roasto, Mati; Kaldmäe, Hedi; Bleive, Uko; Rätsep, Reelika
Ettekandes käsitleme: käimasoleva ResTA 14 projekti TAIMLOOMTOIT ühte suuremat katset •Projekti TAIMLOOMTOIT eesmärgiks o n teaduslikult põhjendatud suurima liha ja kala keemilise ja bakterioloogilise riknemise põhiprotsesside pidurdamise võimega bioaktiivsete lisandite väljaselgitamine taimse tootmise jääkides , nende jääkide võimalikult täielikuks ning eesmärgipäraseks kasutamiseks (Rätsep jt , 2021). •NB! Paljudel juhtudel on taimse tootmise jäägid hoopis rikkamad bioaktiivsete ainete poolest kui põhitooted , näiteks mahlad •Kokku tehti projekti raames 2021. aasta jooksul neli erineva suurusega katset, milleks valiti välja sea hakkliha ja taimsete materjalide segud, mis meie varasemate katsete tulemuste põhjal võisid anda parimaid tulemusi, arvestades erinevaid toime mehhanisme.
Komplekssed kestvuskatsed toidu säilimisaja määramiseks
(Eesti Maaülikool, 2022) Koskar, Julia; Roasto, Mati; Laikoja, Katrin; Meremäe, Kadrin; Rätsep, Reelika
Käesoleva artikli eesmärgiks on anda ülevaade toidukäitlemisega alustavale ettevõtjale toidu säilimisaja määramiseks vajalike kestvuskatsete planeerimisest, sealhulgas laboratoorsete analüüside valiku põhimõtetest ja võimalustest Eestis. Mitmetele toidugruppidele ei ole õigusaktidega kehtestatud toiduohutuse ja protsessi hügieenikriteeriume ega toidu säilitamisnõudeid. Sellisel juhul tuleb need kehtestada toidukäitlejal (Toiduseadus, §22). Kestvuskatse puhul on tegemist toidu valmistaja ja/või pakendaja poolt planeeritud ning laboratooriumis läbiviidud analüüsitegevustega, mille tulemuste põhjal määratakse toidu säilitamisnõuded (sh toidu säilimisaeg ja säilitamistingimused), hinnates toidu ohutust ning toidu vastavust teistele nõuetele (Roasto & Laikoja, 2019). Toidu säilimisaeg on ajaperiood, mille jooksul määratletud säilitamistingi- musi rakendades püsib toit ohutu, säilitab soovitavad kvaliteediomadused ja on inimtoiduks kasutuskõlblik (Roasto & Laikoja, 2019). Kestvuskatsete eesmärk on kinnitada, et toit vastab kogu säilimisaja jooksul nii mikrobioloogilistele, keemilistele kui ka muudele toidule kehtestatud nõuetele. Seega tuleb toidu säilimisaja määramisel arvesse võtta nii toi- duohutuse kui kvaliteedi indikaatoreid ning sageli ka toidu organoleptilisi omadusi.
Listeria monocytogenes’e kasvu pidurdamise võimalused taimsete lisanditega hakklihatoodetes
(Eesti Maaülikool, 2023) Roasto, Mati; Meremäe, Kadrin; Koskar, Julia; Elias, Terje; Rätsep, Reelika
Listerioos on Euroopa Liidus (EL) viies kõige sagedamini registreeritud zoonoos inimestel ja üks tõsisemaid toidutekkelisi haigusi. 2021. aastal vajas EL-s 96,5% listerioosi haigestunutest haiglaravi ning 13,7% listerioosi haigusjuhtudest lõppes surmaga (EFSA, 2022). Listerioosi riskigruppi kuuluvad rasedad, vastsündinud, vanurid ja immuunpuudulikkusega inimesed. 2021. aastal teatati kõige sagedamini L. monocytogenes´e infektsioonidest vanuserühmades „üle 64-aastased“ ja „üle 84-aastased“ (EFSA, 2022). Valmistoitudele1 on EL-s kehtestatud toiduohutuskriteeriumid. Valmistoitudes, milles L. monocytogenes võib paljuneda, ei tohi patogeeni esineda 25 grammis tootes enne selle tootnud toidukäitleja vahetu kontrolli alt väljaviimist (enne toidu turustamist)2. Valmistoitudele, milles L. monocytogenes ei paljune, on kehtestatud arvuline kriteerium 100 pmü/g ehk kogu toidu säilimisaja vältel peab patogeeni arvukus grammi toidu kohta jääma alla 100 bakteri. Juhul, kui toidu valmistaja suudab pädevale asutusele piisavalt tõendada, et kogu säilimisaja jooksul kriteerium 100 pmü/g ei saa ületatud, kuid samas on tegemist toiduga, milles L. monocytogenes võib teatud määral paljuneda, kehtib nõue, et L. monocytogenes´e arvukus ühes grammis valmistoidus ei tohi ületada 100 pmü-d kogu toidu säilimisaja vältel2. Juhul, kui toidukäitleja soovib rakendada valmistoidule, milles L. monocytogenes võib paljuneda, kriteeriumi 100 pmü/g, tuleb teostada nõuetekohane3 nakatamiskatse. Viimane eeldab laboratoorseid analüüse, et hinnata toitu tahtlikult viidud (inokuleeritud) L. monocytogenes´e võimet toidus konkreetsetes säilitamistingimustes ellu jääda ja/või paljuneda (Roasto ja Laikoja, 2019). Tingituna L. monocytogenes´e olulisusest valmistoitude toiduohutust mõjutava tegurina ning toidukäitlejate toiduohutuse tagamise kohustusest4 on vajadus hinnata toidu sisemiste, väliste ja kaudsete tegurite mõju L. monocytogenes´e kasvule selles, eriti valmistoitudes, millel on pikk säilimisaeg. Valmistoite, mille säilimisaeg on kuni 5 päeva, peetakse toitudeks, milles L. monocytogenes ei paljune ohtliku määrani. Käesoleva artikli eesmärgiks on anda ülevaade L. monocytogenes’e kasvu pärssimise võimalustest taimsete lisanditega rikastatud hakklihatoodetes.
Manifestation of Heat-Induced Valuable Dietary Nucleotide Salvage in Food Prepared from Aged Fish in Fast Protein and Metabolites Liquid Chromatography, ATP-Bioluminescence Assay, and NMR Spectra
(MDPI, 2023) Sünter, Alar; Kuznetsov, Artur; Rausepp, Piret; Püssa, Tõnu; Toom, Lauri; Konoplev, Georgii; Stepanova, Oksana S.; Stepanova, Oksana V.; Lyalin, Daniil; Frorip, Aleksandr; Roasto, Mati
Dietary nucleotides and nucleosides, primarily inosine monophosphate (IMP) and the adenine nucleotide pool (ANP), are widely considered as essential nutrients responsible for multiple biological functions. Food prepared from meat and fish is the main source of these substances in the human diet, and it is extremely important to implement storage and processing techniques ensuring their maximum preservation and even accumulation during maturation or conditioning. In experiments with freshly refrigerated grass carp and defrosted Alaska pollock fillets it was discovered, initially using Fast Protein and Metabolites Liquid Chromatography and the ATP- bioluminescence test, and afterwards validated by NMR spectroscopy, that heat treatment identical to conventional culinary processing in aqueous or wet media at temperatures above 62 ◦C leads to nucleotide salvage (recovery) in aged fish. A significant increase in the concentration of IMP, and even an emergence of ANP substances, were reliably demonstrated in fish samples which had already partially or fully lost these components during prolonged storage due to the ATP breakdown metabolic reactions. Owing to this recovery, the nutritive value of ready-to-eat food can be higher than was initially evaluated in raw products before heat treatment: an effect that should certainly be considered in practical nutrition. Moreover, it is necessary to reconsider the widely acknowledged system of indices of freshness based on nucleotides and nucleosides elaborated a long time ago for raw meat and fish products.

Sirvi

Sirvi VLI projektid Pealkiri järgi

Tulemused lehekülje kohta

Sorteerimisvalikud