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2024

Selle valdkonna püsiv URIhttp://hdl.handle.net/10492/9341

Sirvi

Sirvi 2024 Märksõna "Adenostemma platyphyllum" järgi

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    Kirje
    Metabolite profiling, terpenoid and kaurenoic acid production of Adenostemma platyphyllum at different concentrations of hydroponic solutions in the wick system
    (Estonian University of Life Sciences, 2024) Tamsin, A.H.N.; Nurfalah, R.; Trivadila; Batubara, I.; Rafi, M.; Ridwan, T.; Aziz, S.A.; Takemori, H.
    Adenostemma platyphyllum, a medicinal plant belonging to the Asteraceae family, has gained increasing attention due to its potential as a source of bioactive compounds with diverse therapeutic properties but has not been widely cultivated. This work aims to obtain the optimum concentration of AB-mix solution to produce higher terpenoid and kaurenoic acid, as well as metabolite profiling in cultivating A. platyphyllum using a hydroponic wick system. This research uses a one-factor randomized block design of different concentrations of AB-mix nutrient solutions. Total terpenoids were quantified using the UV-Vis spectrophotometric method, total kaurenoic acid was determined using a high-performance liquid chromatography (HPLC) method, and the metabolite profiling was analyzed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) instrument. Several terpenoid compounds have been identified in A. platyphyllum, including Ent-17-Oxo-15-kauren-19-oic acid, andrographolide, cafestol, alphaFarnesene, curcumene, as well as ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic acid (11αOH-KA) and 11α,15-dihydroxy-16-kauren-19-oic acid (11α,15OH-KA), which belong to the kaurenoic acid group. The plants had the highest total terpenoid and kaurenoic acid found in 1,300 mg L-1 nutrient concentrations. On the other hand, the highest terpenoid and kaurenoic acid productivity were found in plants with 900 and 1,300 mg L-1 AB-mix solution, respectively. Therefore, the optimum concentration of nutrient solution to produce optimum terpenoid and kaurenoic acid levels in A. platyphyllum cultivation by hydroponic wick system was 1,300 mg L-1.