Veterinaarmeditsiini ja loomakasvatuse instituut
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Kirje Book of Abstracts XIX Baltic animal breeding conference, December 14-15, 2022, Tartu, Estonia(Estonian University of Life Sciences, 2022) Viinalass, Haldja (compiler); Arney, David (compiler); Tänavots, Alo (compiler); Institute of Veterinary Medicine and Animal SciencesDear colleagues, On behalf of the Organizing Committee, I am pleased to invite you to attend the XIX Baltic Animal Breeding Conference that will be held in Tartu, Estonia, at the Estonian University of Life Sciences from December 14-15, 2022. The recent pandemic has shown us that our sector never stops, that animal production continues to produce food for us and that we are an essential part of society. In 2022 we will surely be under the economic and societal consequences of this pandemic and the changed situation in the world. This year’s Baltic Animal Breeding Conference is already the 19th. The Baltic Animal Breeding Conference, which has taken place over the years, will give us an opportunity to exchange information and new ideas, and will encourage the development of further fruitful collaboration and networking. The conference will present ideas of what animal husbandry researchers and lecturers in the Baltic countries are currently doing. The conference is planned in a traditional way: a plenary session in the morning and a species session in the afternoon on December 14th , followed by a technical tour on December 15 th . The programme will cover various areas of animal production and the topics will be filled with innovation and recent scientific results in animal production. As a community, we must make up for the lost opportunities for face-to-face networking. Happy listening, nice meetings and flying ideas! Prof Haldja Viinalass, Chair of Organizing CommitteeKirje Converging Extracellular Vesicle Research in all domains of life towards understanding their potential functional application : Proceedings of the COMBIVET & OH-BOOST Joint Conference 2023: 8-9th September 2023(Estonian University of Life Sciences, 2023) Estonian University of Life Sciences; Ekanayake, Gayandi (editor); Muhandiram, Subhashini (editor); Godakumara, Kasun (editor); Sapugahawatte, Dulmini N. (editor); Kodithuwakku, Suranga (editor); Fazeli, Alireza (editor)About the COMBIVET & OH-Boost Joint Conference 2023 This conference is jointly organised by the ERA Chair COMBIVET and OH- Boost Twinning Project of the Estonian University of Life Sciences in partnership with the University of Tartu (UT), University of Helsinki (UH) the University of Milan (UniMi) and Baltic Society of Extracellular Vesicles (BSEV). The COMBIVET and OH-Boost programmes are supported by European Union’s Horizon 2020 research and innovation programme under grant agreement No. 857418 COMBIVET and the European Union through Horizon coordination and support actions under grant agreement No. 101079349 OH-Boost respectively.Kirje The effect of pasteurized milk extracellular vesicles on bacterial growth(Estonian University of Life Sciences, 2023) Sapugahawatte, Dulmini Nanayakkara; Midekessa, Getnet Balcha; Godakumara, Kasun; Prasadini, Madhusha; Kodithuwakku, Suranga; Roasto, Mati; Andronowska, Aneta; Fazeli, AlirezaExtracellular vesicles (EVs) are released by cells and have a lipid bilayer structure. EVs harbor various molecules, including proteins, RNAs, and DNAs. Studies of mammalian EVs are increasingly attracting the interest of researchers; however, there are only few studies of nanoparticles in food. Milk-derived EVs can survive high temperatures and digestion process, while retaining their biological activity. This study investigated the effect of pasteurized cows’ milk derived EVs on growth of five different bacteria. Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 53868, Bacillus subtilis, Bacillus cereus and Pseudomonas aeruginosa with a concentration of 1x107 CFU/ml were separately co-cultured with pasteurized milk derived EVs (1x109 EVs/μl) in Muller Hinton broth. The bacterial growth was monitored as absorbance at 620 nm over 24 hours. Bacteria treated with phosphate buffer solution (PBS) were considered negative control throughout the experiment. The percentage bacterial growth difference was COMBIVET & OH-BOOST JOINT CONFERENCE 2023 55 determined with respect to negative control and results expressed as mean ± standard error of mean. All analyses were performed in three biological triplicates and each biological replicate consisted of three technical replicates. Co-culture of bacteria with milk EVs demonstrated that EVs could decrease the growth of S. aureus, B. subtilis, B. cereus and P. aeruginosa. Highest growth inhibition was observed for B. subtilis (33.9% ± 2.4) followed by B. cereus (18.1% ± 3.7) at 6 hours of incubation. S. aureus and P. aeruginosa growth were inhibited by 12.9% ± 1.3 and 9.9% ± 3.5 respectively after 9 hours incubation. Only the S. aureus growth inhibition at 9 hours was statistically significant (P=0.022) according to T-test. However, E. coli growth was not affected by milk EVs compared to the control. In conclusion, the dietary EVs can be absorbed by bacteria and pasteurized milk derived EVs has a selective inhibitory activity on the growth of some bacteria.Kirje Growth potential of L. monocytogenes in ready-to-eat minced meat samples enriched with plant powders(Faculty of Pharmacy, University of Helsinki, 2023) Koskar, Julia; Roasto, Mati; Meremäe, Kadrin; Elias, Terje; Mäesaar, Mihkel; Rätsep, ReelikaListeriosis is the fifth most frequently recorded human zoonosis in the European Union and one of the most serious foodborne diseases. Regulation (EC) No 2073/2005 lays down the microbiological criteria for Listeria monocytogenes in ready-to-eat (RTE) foods. In case a food business operator wants to apply the numeric criterion of 100 cfu/g to RTE food, a challenge test needs to be performed in accordance with the European Union Reference Laboratory for Listeria monocytogenes (EURL Lm) technical guidance document on challenge tests and durability studies regarding L. monocytogenes. The aim of this research is to provide an overview of the possibilities of inhibiting the growth of L. monocytogenes in minced meat products enriched with plant powders.Kirje Proceedings of 2nd annual Baltic Society of Extracellular Vesicles conference 2023 : April 28-29, 2023(Estonian University of Life Sciences, 2023) State Research Institute Centre for Innovative Medicine; Pilipenko, Vladimirs (editor); Midekessa, Getnet (editor); Pivoriunas, Augustas (editor); Fazeli, Alireza (editor)The Baltic Society of Extracellular Vesicles (BSEV) Conference is organized at the State Research Institute Centre for Innovative Medicine. BSEV has set an objective to bring prominent experts in the field to exchange the most recent knowledge in this growing field and advance research cooperation within Baltic states.Kirje Proceedings of the first Combivet and Baltic Society of Extracellular Vesicles joint conference 2022 : 30th September & 1st of October, 2022(Estonian University of Life Sciences, 2022) Estonian University of Life Sciences; Muhandiram, Subhashini (editor); Kodithuwakku, Suranga (editor); Godakumara, Kasun (editor); Fazeli, Alireza (editor)About COMBIVET & BALTIC SOCIETY OF EXTRACELLULAR VESCILES JOINT CONFERENCE 2022. The Combivet and Baltic Society of Extracellular Vesicles (BSEV) Joint Conference is organized by the COMBIVET ERA Chair of Comparative Medicine in the Institute of Veterinary Medicine and Animal Sciences of the Estonian University of Life Sciences and Baltic Society of Extracellular Vesicles. The program is supported by the European Union (Horizon 2020 research and innovation programme under grant agreement No 857418).Kirje Unlocking the potential of extracellular vesicles in one-health, food and feed : Proceedings of the COMBIVET & OH-BOOST Joint Conference 2024: 24-25th of May 2024 : abstract book(Estonian University of Life Sciences, 2024) Estonian University of Life Sciences; Premathilka, Chanaka (editor); Ekanayake, Gayandi (editor); Sapugahawatte, Dulmini N. (editor); Kodithuwakku, Suranga (editor); Fazeli, Alireza (editor)This conference is jointly organised by the ERA Chair COMBIVET and OHBoost Twinning Project of the Estonian University of Life Sciences in partnership with the University of Tartu (UT), University of Helsinki (UH) the University of Milan (UniMi) and Baltic Society of Extracellular Vesicles (BSEV). This conference marks the final gathering under the guidance of the ERA Chair COMBIVET. The COMBIVET and OH-Boost programs are supported by the European Union's Horizon 2020 research and innovation programme under grant agreement No. 857418 COMBIVET and the European Union through Horizon coordination and support actions under grant agreement No. 101079349 OH-Boost respectively.