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2. Magistritööd

Selle kollektsiooni püsiv URIhttp://hdl.handle.net/10492/2498

Sirvi

Sirvi 2. Magistritööd Märksõna "air quality" järgi

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    Kirje
    Microbiological composition of the air in dairy farms using different types of bedding
    (Eesti Maaülikool, 2023) Välipakka, Emilia Anna Sofia; Aland, Andres; Kaasik, Allan; Praakle, Kristi
    Bedding material is one of the fundamental elements of animal housing environment, as animals like to spend a significant amount of time on it. The main interest in previously published studies concerning bedding materials has been in laying comfort, milking performance, and stall hygiene. Surprisingly, the present understanding of the bedding material's effect on the microbiological quality of the air is scarce. This study aimed to compare microbiological air quality in two free-stall dairy cattle farms using different kinds of bedding materials – peat and sawdust. Both of the study farms (Farm A = peat; Farm B = sawdust) were visited three times from spring 2022 to winter 2023. The air samples were collected by using two different methods: sedimentation and filtration. In total, 30 sedimentation samples and 72 filtration samples were collected. To apply the sedimentation method, five agar plates were placed on a polystyrene box at a height of one metre from the floor surface, after which all plates were opened for a specified time. Filtration samples were collected by using a portable air sampler (Sartorius AirPort MD8). The air samples were taken to the microbiology laboratory and cultivated on four types of growth media (Columbia agar, Columbia blood agar, MacConkey agar, and Sabouraud dextrose agar) to identify mesophilic bacteria, pathogenic bacteria, Gram-negative and enteric bacteria, and also fungal species. According to the results of the study, the two investigated bedding materials caused different microbial composition of the air. The results indicate that the bedding material may substantially affect the microbial composition of the air and thus the quality of the animal housing environment, but the field needs relevant studies more.