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2. Magistritööd

Selle kollektsiooni püsiv URIhttp://hdl.handle.net/10492/2498

Sirvi

Sirvi 2. Magistritööd Märksõna "acute phase proteins" järgi

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  • Pisipilt
    Kirje
    The dynamics of Cryptosporidium spp. during first three week of calves’ life and the effect on the general inflammatory response
    (Eesti Maaülikool, 2022) Erling, Dimi; Dorbek-Kolin, Elisabeth
    Cryptosporidium spp. infection is highly prevalent in Estonian and worldwide on herd level. Most infected group is young neonatal calves who also shed oocysts from early age. Cryptosporidium spp. can cause severe enteritis, diarrhea, and increased mortality along with economical losses. This thesis focuses on the dynamics of Cryptosporidium spp. infection on one Estonian dairy farm, the inflammatory response of the neonatal calf, and if and how the acute phase proteins (APP) associate with Cryptosporidium spp. infection. During infection, the innate immune system acute-phase response (APR) is activated and as a result APPs are produced. The inflammatory response in calves was assessed by measuring the major cattle APP of cattle, serum amyloid A (SAA) and haptoglobin (Hp). From the study group of calves (n=55), serum (n=264) and fecal (n=220) samples were taken from one Estonian dairy farm at 7, 10, 14 and 21- days of age. Oocyst groups were formed retrospectively, where G0 group had no oocysts, G1 1-10.000 oocysts and G2 over 10.000 oocysts. Majority of calves at 14- day of age belonged to the group G2. Mean SAA concentration peaked at the 7-day old calves, from where it steadily declined. Mean Hp concentration peaked at day ten and during that time majority of the calves (70.9%) belonged to the G2 group. The result could indicate that high numbers of Cryptosporidium spp. oocysts could influence the calves’ immune system and promote APR that is more pronounced in Hp than in SAA.
  • Pisipilt
    Kirje
    The dynamics of Rotavirus during first three weeks of calves’ life and the effect to the inflammatory response
    (Eesti Maaülikool, 2024) Kolga, Kert; Niine, Tarmo (advisor)
    Bovine rotavirus (BRV) infection is a zoonotic infection that is prevalent worldwide, has extensive implications on the health of calves, and has a major negative economic impact. During BRV infection, the acute phase response (APR) could be activated. Blood (n = 313) and fecal (n = 308) samples were collected from 63 dairy farm calves at 1, 7, 10, 14, and 21 days of age. Acute phase protein (APP) markers, serum amyloid A (SAA), and haptoglobin (Hp) concentrations were measured in serum samples, whereas the presence of rotavirus antigen was assessed in feces. Groups were retrospectively categorized based on age and the presence of rotavirus antigen. Of the 63 calves studied, 31 (61.9%) tested positive for rotavirus at some point of the study, with the highest positivity rate (41%) observed in the 10-day old calves. The mean SAA concentration peaked in 7-day old calves (156.1 mg/L). Mean Hp blood serum concentrations showed two peaks in 1-day old calves and 10-day old calves, at 216.2 mg/L and 219.5 mg/L, respectively. A statistically significant association (p = 0.016) between Hp and rotavirus infection was observed using a logistic regression model. Although the results suggest a significant association between rotavirus infection and elevated Hp blood serum concentration, further research is warranted to validate these findings.