SEARMET
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Kirje Low molecular weight metabolites as possible new non-invasive tool for selecting bovine in vitro produced embryos(2017) Nõmm, Monika; Porosk, Rando; Pärn, Pille; Soomets, Ursel; Jaakma, Ülle; Kõks, Sulev; Kilk, KalleSelecting high quality preimplantation embryo for transfer has been the most difficult task when producing embryos in vitro. To date the most used non-invasive method is based on visual observation. Developing a non-invasive method for embryo assessment is essential to have a profitable in vitro embryo production (IVP) and embryo transfer system. Molecular characterization of embryo growth media has been proposed as an complementary method to visual assessment of embryo morphology. In this study we are demonstrating a novel method, allowing sample collection at different embryo development stages, without compromising embryo quality, to determine potential viability markers for bovine IVP. Single bovine embryos were cultured in 60µl SOF+0.4% BSA droplets under mineral oil. Twenty µl of culture media was removed at day 2, 5 and 8 post-fertilization. A total of 58 samples were analyzed using liquid chromatography-mass spectrometry (Q-Trap 3200), followed by principal component analysis. Our results indicate that there are significant differences (p<0,00001) in concentrations for proline (m/z = 116), inositol (m/z of sodium adduct = 203) and citrate (m/z of sodium adduct = 215) also in the amino acid group of leucine and isoleucine (m/z = 132), phenylalanine (m/z = 165) and arginine (m/z = 211) between the normally developed and retarded in development embryo culture media. Platelet activating factor (m/z = 524) (PAF) was roughly 3 fold increased in day 5 to day 8 embryo culture media. Unfortunately the increase of PAF was not statistically significant between normally developing and retarded embryos. These results demonstrate that it is possible to remove culture media samples from droplets and not significantly affect embryo development. Applying this method for embryo selection provides a possibility to identify well-developing embryos and provides an opportunity for improving the herds genetic value.Kirje Animal reproduction, technology and welfare, December 3-4, 2018, Estonian University of Life Sciences, Tartu, Estonia : [poster](2018) Estonian University of Life SciencesPoster of the final conference of the SEARMET "Animal reproduction, technology and welfare".Kirje "In vitro 3-D total guidance and fitness" proceedings of the CellFit workshop 2018 and "Intercellular epigenomics" SEARMET and TRANSGENO joint workshop 2018 : Tartu, Estonia 10-11 April, 2018(Estonian University of Life Sciences; The COST Action CA16119, 2018) Estonian University of Life Sciences; Jaakma, Ülle (editor); Przyborski, Stefan (editor); Rimann, Markus (editor); Brevini, Tiziana (editor); James, Victoria (editor); Fazeli, Alireza (editor)About the European Co-operation in Science and Technology. The European Cooperation in Science and Technology (COST) is the oldest and widest European intergovernmental network for cooperation in research. Established by the Ministerial Conference in November 1971, COST is presently used by more than 30,000 scientists of 35 European countries to cooperate in common research projects supported by national funds. The financial support for cooperation networks (COST Actions) provided by COST is about 1.5% (30 million EUR per year) of the total value of the projects (2,000 million EUR per year). The main characteristics of COST are: bottom up approach (the initiative of launching a COST Action comes from the European scientists themselves); à la carte participation (only countries interested in the Action participate); equality of access (participation is also open to the scientific communities of countries which do not belong to the European Union) and flexible structure (easy implementation and management of the research initiatives). As aprecursor of advanced multidisciplinary research, COST has a very important role in shaping the European Research Area (ERA). It anticipates and complements the activities of the current Framework Programme for Research and Innovation (Horizon 2020). COST activities create a bridge between the scientific communities of countries and increases the mobility of researchers across Europe in many key scientific domains.Kirje Animal reproduction, technology and welfare, December 3-4, 2018, Estonian University of Life Sciences, Tartu, Estonia : [speakers at SEARMET conference](2018) Estonian University of Life SciencesSpeakers at the final conference of the SEARMET "Animal reproduction, technology and welfare".Kirje Animal reproduction, technology and welfare, December 3-4, 2018, Estonian University of Life Sciences, Tartu, Estonia : [presentations](2018) Hyttel, Poul; Proudfoot, Christopher; Arney, David; Guo, Yongzhi; Salumets, Andres; Chankeaw, Wiruntita; Jaakma, Ülle; Mark, Elina; Strøbech, Lotte Bjørg; Ivask, Marilin; Holt, William; Loi, Pasqualino; Morrell, Jane; Tagoma, Aili; Thurston, Lisa; Reinsalu, Olavi; Nõmm, MonikaPresentations of the final conference of the SEARMET "Animal reproduction, technology and welfare".Kirje Animal reproduction, technology and welfare, December 3-4, 2018, Estonian University of Life Sciences, Tartu, Estonia : [photos](2018) Koemets, Mari-Liis (photographer); Nõmm, Monika (photographer); Ivask, Marilin (photographer)Photos of the final conference of the SEARMET "Animal reproduction, technology and welfare".Kirje Animal reproduction, technology and welfare, December 3-4, 2018, Estonian University of Life Sciences, Tartu, Estonia : [program](2018) Estonian University of Life SciencesProgram of the final conference of the SEARMET "Animal reproduction, technology and welfare".