Sirvi Autor "Tsytsiura, Y." järgi
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Kirje Assessment of the relation between the adaptive potential of oilseed radish varieties (Raphanus sativus l. var. oleiformis Pers.) and chlorophyll fluorescence induction parameters(Estonian University of Life Sciences, 2023) Tsytsiura, Y.The possibility of optimization of the system of varietal identification, particularly of cruciferous crops in the breeding programs an urgent task that needs a scientific solution. A system comparison of a complex model for assessing genotypes for plasticity and stability with indicative basic and derivative indicators of the chlorophyll fluorescence induction curve (CFI) is proposed as a possible solution to this problem. 14 varieties of oilseed radish of different ecological and geographical origin were chosen as the object of research. Widely tested indicators of both methodological approaches were applied, taking into account the agrotechnological aspects of the analysis of oilseed radish plants for uniformity and stability. The varietal specificity of the basic indicators of the induction of chlorophyll fluorescence of oilseed radish in response to changes in the stress level of the years of the research period was established. The determined interval of correlation-regression dependencies at the level of -0.382–(-0.658) (p < 0.05–0.01) between the indicators of genotypic stability (Gp), adaptability (bi), and selection value (Sv) and the basic indicators of the CFI curve such as minimal fluorescence (F0), fluorescence of the 'plateau' zone (Fрl), maximal fluorescence (Fm) and fluorescence stationary level (Fst). Direct correlations were determined at the level of 0.652–0.745 (p < 0.01–0.001) in the same comparison system for maximal fluorescence (Fm), fluorescence rise (dFpl), maximum variable fluorescence (Fv), photochemical efficiency (EP), leaf water potential (Lwp), plant viability index (RFd), efficiency of the initial reactions of photosynthesis (Kprp), coefficient of decline of the fluorescence (Kfd). The indicated pair correlation dependences were confirmed by the results of multiple regression analysis for the value of multiple regression coefficients (R) in the interval of 0.793–0.833 (p < 0.05–0.01). On the basis of the obtained data, an integrated version of the assessment of varieties of cruciferous crops is proposed, which allows optimization of the system of varietal identification, particularly of cruciferous crops, in the breeding programs of their pre- and post-registration study, as well as the system of searching for donors and sources of relevant traits in the breeding hybridization programs at various levels. In terms of further research, it will be promising to apply this variant of the analysis to wild species of cruciferous crops in a single complex with their cultural forms to assess the regularities of the formation of adaptations under the directed trait selection process.Kirje Chlorophyll fluorescence induction method in assessing the efficiency of pre-sowing agro-technological construction of the oilseed radish (Raphanus sativus L. var. oleiformis Pers.) agrocenosis(Estonian University of Life Sciences, 2022) Tsytsiura, Y.Chlorophyll fluorescence induction (CFI) is a measure of photosynthetic performance and is widely used by plant physiologists and ecophysiologists. The basic principle of CFI analysis is relatively straightforward. The specified method of analysis during 2015–2020 was applied to assess the optimality of selection of technological sowing parameters such as sowing rate (estimated interval 0.5–4.0 million germinable seeds ha-1 ), row width (15–30 cm), presowing fertilizer (N0–90P0–90K0–90) for three varieties of oilseed radish. The widely tested basic indicators of the CFI curve (F0, Fpl, Fm, Fst) were used, as well as possible indices and ratios calculated on their basis in accordance with the CFI analysis methodology. For the first time, the species characteristics of oilseed radish were investigated by the nature of the CFI curve in relation to spring rape, white mustard, and spring mustard on the 1.5 germinable seeds ha-1 (30 cm row width, N0P0K0) variant. It was established by the stress sensitivity category of the PSII photosystem that a reliable possibility of using the CFI method for identification studied technological options for sowing. The share of the influence of the technological factor of the sowing method (in %) on the formation of indicators F0, Fpl, Fm, Fst in the dispersion scheme of the experiment was consistently 19.3, 8.4, 19.5, 6.3. The influence of the seeding rate factor on the results of F0, Fpl, Fm, Fst was (in %) 26.6, 9.5, 42.3, 9.3 and the influence of the fertilizer factor was 13.5, 16.4, 5.7, 12.7, respectively. The formation of the specified basic indicators of the CFI curve in the resulting interaction of the technological parameters of sowing depended on the hydrothermal conditions of the vegetation of oilseed radish with the share of influence of 20.1, 40.2, 28.1, 30.0, respectively. It was determined that the decrease in the indicator of the hydrothermal coefficient (in the ratio of the increase in the sum of average daily temperatures to the decrease in the amount of precipitation) ensures the following dynamics of changes in the main and derivative indicators of CFI: a decrease Fpl 1.3%, Fm 11.8%, ER 8.7%, Lwp 15.9%, RFd 25.3%, Kprp 21.9%, Kfd 17.7% and growth F0 5.1%, Fst 7.3%, Que 40.4%, Kef 24.0%, Vt 71.3%. The comparison during the study period of options 4.0 and 0.5 million germinable seeds ha-1 determined an averaged decrease in F0 and Fst indicators by 29.5% and 29.1% while increasing Fpl and Fm by 2.2% and 38.5%. According to the determined level of CFI indicators for various technological schemes of sowing, an expedient option was recommended, which ensures the highest efficiency of the PSII photosystem of oilseed radish in the range of 1.0–2.0 germinable seeds ha-1 with a fertilization rate of N30–60P30–60K30–60 for row sowing and 1.5 germinable seeds ha-1 with a fertilization rate of N60–90P60–90K60–90 for wide-row sowing.Kirje Development of an integrated soil quality index under prolonged green manure application of oilseed radish in crop rotation(Estonian University of Life Sciences, 2026) Tsytsiura, Y.Over a 12-year study period, the effectiveness of using intermediate green manuring with oilseed radish (Raphanus sativus L. var. oleiformis Pers.) – applied once every two years – was evaluated under conditions of repeated application in the same field within a crop rotation system (including green manuring of crops such as grain sorghum (Sorghum bicolor L.), pea (Pisum sativum L.), soybean (Glycine max (L.) Merrill), sunflower (Helianthus annuus L.), chickpea (Cicer arietinum L.) and maize (Zea mays L.), with three assessment checkpoints in 2014, 2019, and 2025. The results showed that the green-manured treatment, averaged across the entire evaluation period, produced positive changes in the physical and chemical soil fertility parameters within the 0–30 cm soil layer compared with the unfertilized control. Increases were observed in humus content (by 24.65%), water absorption capacity (by 30.04%), easily hydrolyzable nitrogen (by 33.67%), available phosphorus (by 25.72%), exchangeable potassium (by 23.10%), and total porosity (by 25.04%). Decreases were recorded in bulk density (by 19.05%), particle density (by 9.95%), soil hardness (by 33.95%), and pH (by 5.60%). Green manuring also contributed to optimizing the proportion of humic acids in the humus structure by a factor of 1.2 and to achieving a total organic carbon to total nitrogen ratio of 10.36:1, representing an 11.92% increase compared with the control. As a result, the application of green manuring was reflected in an improved integrated Soil Fertility Index, with a value of 0.692 compared with 0.499 in the non-manured control.Kirje Estimation of biomethane yield from silage fermented biomass of oilseed radish (Raphanus sativus l. var. oleiformis Pers.) for different sowing and harvesting dates(Estonian University of Life Sciences, 2023) Tsytsiura, Y.The potential possibility of using oilseed radish biomass of different sowing dates (technological interval from spring to summer (post-harvest) sowing) and phenological harvesting (budding-green pod) after the silage fermentation procedure for the production of biogas and biomethane using the methodology of anaerobic digestion with the addition of inoculum was investigated. Comprehensive methods for assessing the kinetics and dynamics of production of both total biogas volumes and biomethane production levels were applied, with an assessment of the levels of approximation of the curves of daily volume fixation. Based on a systematic analysis with an assessment of the individual characteristics of the kinetics of the process of anaerobic digestion of silage substrate for each variant of the experiment, the high potential of this crop at different terms of its sowing and harvesting with a level of bioproductivity at the level of 0.54–3.62 t ha-1 DM (depending on the phase and timing of sowing) at the level of biomethane productivity in the range of SMY 201.03–319.66 LN kg-1 ODM at the level of biomethane concentration in the range of 49.92–59.11%. The maximum level of biomethane production was achieved when using silage mass (inoculum subtracted) obtained by silage fermentation from fresh biomass harvested in the flowering phase during the first sowing period with a level of biochemical methane potential (BMPGomp) of 344.13 LN kg-1 ODM, specific methane yield (SMY) of 319.66 LN kg-1 ODM at a maximum specific methane production rate (Rm) of 33.74 LN kg-1 ODM d-1.Kirje Formation and determination of the individual area of oilseed radish leaves in agrophytocenosises of different technological construction(2020) Tsytsiura, Y.For the successful control of the production process, determining the leaf area is a basic requirement. In this context, it is important to determine the regularities of leaf formation within the plant, considering technological parameters of agrophytocenosis construction. These are the important issues covered in this paper based on the years of research conducted between 2013 and 2018 on three cultivars of oilseed radish: one of the poorly explored members of the cruciferous family of multipurpose use. The conducted researches allowed to distinguish features of longline leaf formation of oilseed radish cultivars and mathematically describe features of formation of their area, length and width at the early flowering phase according to the Richards growth curve. The peculiarities of formation of individual leaf area depending on the combination of the variations of the stand density and fertilization in the context of the recommended process regulation of oilseed radish cultivation are also determined. It has been proved possible to use a non–destructive method of determining the individual leaf area of oilseed radish, basing on the evaluation of 29 models, using the following formula S = 7.9316– 2.3613L + 0.6897 (LW)+0.0458L2–0.0005 (LW)2 (under the following test parameters of the model: R2 0.9106; RMSE 9.75; d 0.956; BIAS –0.1523).Kirje Formation of the root system of oilseed radish under long-term green manuring practices(Estonian University of Life Sciences, 2025) Tsytsiura, Y.The optimal selection of plant species for green manuring technologies is impossible without assessing the bioproductivity and morphometry of their root systems, which is an additional and significant factor in the effectiveness of both the growth processes of the green manure crop and its projected impact on the soil profile as a whole. Taking this into account for 11-year period, the bioproductivity of the root system of oilseed radish was assessed by morphological and weight parameters in the soil profile for two sowing dates of green manure: spring and summer. A wide range of methods based on Profile Wall, Monolith Method and Root maps of a profile wall were used to obtain the main functional indicators of the formation and spatial development of the root system. It was determined that oilseed radish from the point of view of formation of morphological characteristics of the root system provided the yield of root biomass in dry matter of 1.19–1.77 t ha-1 in active interaction with the soil profile 40–80 cm deep with the formation of the following components of the root system morphotype in the range of long-term average values for sowing dates: average diameter of the root taproot 5.13–5.67 mm at its taper 0.55–0.66, volume 26.92–39.09 cm3, volume of root spreading zone 36.75–66.39×103 cm3, fractal dimension (D) 0.63–0.73, maximum root depth 47.36–64.41 cm, fraction of total root mass to the soil depth (0–30 cm) 0.77–0.79. The dependence of the formation of indicators of bioproductivity of the root system with a direct nature on the amount of precipitation (determination 47.19–50.20%), water reserves in the soil (27.04–65.61%) and an inverse nature on the average daily temperature (21.44–25.70%), Vysotsky-Ivanov humidification coefficient (57.30–65.45), De Martonne Aridity Index (47.75–51.12) and soil hardness (37.21–59.29%) was determined.Kirje The influence of agroecological and agrotechnological factors on the generative development of oilseed radish (Raphanus sativus var. oleifera Metzg.)(Estonian University of Life Sciences, 2022) Tsytsiura, Y.During the eight-year research period, we determined the peculiarities and regularities of morphological (length and diameter) and anatomical (stem thickness) features of oilseed radish pods considering their location within the generative part of plants for different types of spatial structure of the inflorescence generated in agrocenosises of different densities. We carried out the analysis and statistical grouping of morphological features of oil radish pod in the full range of possible technological options of pre-sowing construction of its agrocenosises, as well as within the selected three zones (tiers) of inflorescence by the nature of variation and variability of morphoparameters pod, namely, lower, middle and upper. We described in detail the stages of pod formation (microstages ВВСН 69-87) considering features of its linear and radial growth, peculiarities of formation of general internal anatomical structure with analysis of mathematical and statistical regularities of changes in these parameters in accordance as per order of its placement within an inflorescence (separately main axis and system of lateral branches). We determined the optimum technological intervals for the construction of oilseed radish agrocenosises, which ensure the combination of appropriate levels of morphometry formation of its fruit elements with the predicted level of reproductive effort and seed productivity. We made a general assessment of the peculiarities of formation of pod technological effectiveness in terms of ease of threshing and possible losses of seeds depending on the complex of factors under study.Kirje Potential of oilseed radish (Raphanus sativus l. var. oleiformis Pers.) as a multi-service cover crop (MSCC)(Estonian University of Life Sciences, 2024) Tsytsiura, Y.The possibility of oilseed radish use as multi-service cover crop (MSCC) during the ten-year period for spring and summer sowing was investigated. A comprehensive approach to assessing the formation of aboveground and root biomass by biochemical analysis with a comprehensive assessment of the factors that determine the quality, manufacturability and agricultural value of the crop was methodically applied. The actual agricultural value from the point of view of the possible use of oilseed radish as a cover, intermediate, green manure, fodder crop and an additional source for biogas production was analyzed. A multi-year data set based on 8 indicators of the formed plant mass, 17 basic indicators of biochemical composition, and 12 derived indicators of ratios and accumulation was formed. Based on the criterion evaluation and comparison of the long-term data set with similar indicators for cruciferous species used as a multi-service cover crop, oilseed radish was classified as a crop with high adaptive bioorganic potential. This was confirmed by the application of the Multi-criteria decision aiding (MCDA) method. The use of this method proved the possibility of multi-purpose use of oilseed radish as a multi-service cover crop on soils with medium fertility potential for unstable moisture conditions. The order of increasing importance of the direction of critical use of oilseed radish in the spring sowing period was: ‘Catch crop’ (Consistency index 0.188) - ‘Biogas’ (0.226) - ‘Fodder’ (0.370) - ‘Green manure’ (0.340) - ‘Cover crop’ (0.431). A similar order for the summer sowing period was: ‘Cover crop’ (0.244) - ‘Catch crop’ (0.305) - ‘Biogas’ (0.357) - ‘Fodder’ (0.407) - ‘Green manure’ (0.415).
