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Sirvi Autor "Praakle, Kristi (juhendaja)" järgi

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  • Pisipilt
    Kirje
    Effects of cobalt nanoparticles on Microsporum canis in vitro
    (Eesti Maaülikool, 2022) Granholm, Heidi Enneli; Praakle, Kristi (juhendaja); Rauwel, Erwan (juhendaja)
    Dematophytosis is a common infectious skin disease caused by various fungi of the Microsporum, Trichophyton and Epidermophyton species. Microsporum canis (M. canis) is causing dermatophytosis in small animals, and it is a zoonotic disease. Treatment of dermatophytosis ensures milder clinical signs and faster recovery. Cobalt nanoparticles (Co NPs) are currently being researched in order to find therapeutic uses, such as anti-cancer, anti-microbial and anti-fungal properties. The aim of this thesis was to study the antifungal effect of cobalt nanoparticles on the growth of M. canis. Our study was a laboratory study carrying out experiments in vitro. Mixtures of different concentrations of Co NPs with Aloe vera gel and Phosphate Buffered Saline solution were created to test the antifungal properties against dermatophyte M. canis. The results showed that where Co NPs were used, there was no growth of M. canis. The Co NPs are exhibiting an antifungal effect and more research should be done investigating further and on the toxicity. Co NPs could possibly be considered a future treatment of dermatophytosis caused by M. canis.
  • Pisipilt
    Kirje
    Metitsilliiniresistentse Staphylococcus pseudintermedius’e esinemine Eesti varjupaikades peetavatel koertel
    (Eesti Maaülikool, 2024) Kivimägi, Rauno; Praakle, Kristi (juhendaja)
    Metitsilliiniresistentne Staphylococcus pseudintemedius (MRSP) on ülemaailmse tähtsusega bakteriaalne patogeen, mis võib põhjustada koertel mitmesuguseid ravile halvasti alluvaid infektsioone, levida asümptomaatiliselt ning põhjustada loomakliinikutes nosokomiaalset nakkust. Kuigi MRSP on peamiselt koertel esinev patogeen, on seda isoleeritud ka inimestelt, kellel on olnud kokkupuude patogeeni kandvate koertega, sealhulgas koerte omanikelt ja veterinaaridelt. MRSP esinemise uurimiseks Eesti loomade varjupaikades peetavatel koertel viidi läbi ülevaateuuring 2023. aasta märtsis ja aprillis. Uuringusse kaasati 37 koera neljast Eesti varjupaigast, kellelt võeti kokku 74 proovi. Igalt koeralt võeti steriilse vatitampooniga kaks proovi: üks proov võeti nina piirkonnast ja suu limaskestalt ning teine proov võeti perianaalpiirkonnast. MRSP isoleerimine ja identifitseerimine viidi läbi modifitseeritud Euroopa Liidu Referentslaboratooriumi antibiootikumiresistentsuse protokolli abil Eesti Maaülikooli veterinaarmeditsiini ja loomakasvatuse instituudi mikrobioloogia laboris. Kõik 74 tampooniproovi osutusid MRSP suhtes negatiivseks. Saadud tulemused on kooskõlas teiste Euroopa riikide uuringutega, mis viitavad madalale esinemissagedusele. Autori teada on see esimene uuring, mis keskendub MRSP esinemisele Eestis, mis näitab siiski vajadust edasiste uuringute järele.
  • Pisipilt
    Kirje
    Microbiological composition of the air in dairy farms using different types of bedding
    (Eesti Maaülikool, 2023) Välipakka, Emilia Anna Sofia; Aland, Andres (juhendaja); Kaasik, Allan (juhendaja); Praakle, Kristi (juhendaja)
    Bedding material is one of the fundamental elements of animal housing environment, as animals like to spend a significant amount of time on it. The main interest in previously published studies concerning bedding materials has been in laying comfort, milking performance, and stall hygiene. Surprisingly, the present understanding of the bedding material's effect on the microbiological quality of the air is scarce. This study aimed to compare microbiological air quality in two free-stall dairy cattle farms using different kinds of bedding materials – peat and sawdust. Both of the study farms (Farm A = peat; Farm B = sawdust) were visited three times from spring 2022 to winter 2023. The air samples were collected by using two different methods: sedimentation and filtration. In total, 30 sedimentation samples and 72 filtration samples were collected. To apply the sedimentation method, five agar plates were placed on a polystyrene box at a height of one metre from the floor surface, after which all plates were opened for a specified time. Filtration samples were collected by using a portable air sampler (Sartorius AirPort MD8). The air samples were taken to the microbiology laboratory and cultivated on four types of growth media (Columbia agar, Columbia blood agar, MacConkey agar, and Sabouraud dextrose agar) to identify mesophilic bacteria, pathogenic bacteria, Gram-negative and enteric bacteria, and also fungal species. According to the results of the study, the two investigated bedding materials caused different microbial composition of the air. The results indicate that the bedding material may substantially affect the microbial composition of the air and thus the quality of the animal housing environment, but the field needs relevant studies more.
  • Pisipilt
    Kirje
    The occurrence of methicillin-resistant Staphylococcus aureus in the environment of an equine clinic
    (Eesti Maaülikool, 2023) Salmela, Hanna; Praakle, Kristi (juhendaja); Corrêa, Felipe (juhendaja)
    Methicillin-resistant Staphylococcus aureus (MRSA) is a zoonotic pathogen of global importance that can be found in asymptomatic carriers (both animals and humans) as well as on various instruments, equipment, and surfaces in veterinary clinics. The equine clinic environment is considered a potential reservoir and an infection route for MRSA, increasing the risk of infection in both horses and people working with them. Within the scope of this thesis, a descriptive study was conducted to examine the occurrence of MRSA in an equine clinic located in Estonia. A total of 50 environmental swab samples were examined in March 2023 from the environment of an equine clinic. Sampling areas were categorized into human and/or animal contact areas. Isolation and identification of MRSA from the environment samples of the equine clinic were performed in the microbiology laboratory of the Estonian University of Life Sciences according to the EURL-AR modified laboratory protocol. All samples taken from the equine clinic environment were negative for MRSA. To our knowledge, no previous data exist on the occurrence of MRSA in equine clinics in Estonia. The results were as expected considering the low prevalence of MRSA in horses in Estonia, but this study does not completely exclude the presence of MRSA in the clinic environment. Further studies, such as air sampling and evaluation of the biosafety protocols of the clinic could provide useful information for the future.