Sirvi Autor "Nsengumuremyi, D." järgi
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Kirje The effect of sapropel extracts on microflora and physicochemical parameters of Dried Distillers’ Grain(2018) Nsengumuremyi, D.; Barakova, N.V.; Romanov, V.A.; Mityukov, A.S.; Guzeva, A.V.This article is devoted to the effect of ultradispersed humate sapropel extracts, obtained from air - dry samples of sapropel, from Seryodka deposit (Pskov region, Russia) by alkaline extraction under the action of ultrasonic radiation, on microbial and phys icochemical parameters of Distillers’ grain (DG) during storage. At the end of the distillation , wet DG was divided and treated with sapropel extract. The untreated sample served as a control. Both (treated and untreated ) were then dried to 10% moisture content . Microbial and physicochemical parameters (moisture content, pH, titrable acidity (TA), acid value (AV) of fats) were assessed before storage of dried DG. A similar experiment was repeated after one week of storage but on microbial load. The microb ial counts ranged from 2.3 × 10 4 CFU g 1 (untreated) to 1.77 × 10 4 CFU g - 1 (treated) before storage while 2.5 × 10 4 CFU g - 1 to 2.18 × 10 4 CFU g - 1 accounted for after a week of storage. The pH had increased from 4.5 ± 0.1 to 6.1 ± 0.1 before and after treatm ent respectively. TA likewise decreased from 3.2 ± 0.4 to 2.03 ± 0.06%. The results showed that sapropel extracts had effects on microflora and physicochemical parameters of DDG.Kirje Enrichment of the grains from rye wort after shock-activator-disintegrating processing(2019) Sabirov, A.A.; Barakova, N.V.; Nsengumuremyi, D.; Samodelkin, E.A.In this study, the mode of obtaining grains (pellets) from wort prepared from rye processed by the shock-activator-disintegrator (SAD), has been developed. Additionally, the enrichment of the grains by proteins using the strain of yeast producing proteins was carried out. For cultivation of a pure culture of a strain of yeasts producing proteins, grains with a concentration of 43.00 g 100 g -1 reducing substances and 62.71 mg g -1 of the total amount of amino acid were used. Different concentrations (10%, 20%, 30% and 40% by weight of the grains) of filtrate 24.90% dry matter, 21.00 g 100 g -1 reducing substances and 10.82 mg g -1 of the total amount of free amino acid was added to the grains. As nitrogen and phosphorus-containing mineral feed, diammonium phosphate was added to the nutrient medium. To obtain a pure culture of Candida tropicalis, SK-4 yeast strain was isolated. The content of crude protein and the concentration of amino acids were determined. The morphological state of the cells was assessed. The results of this study show that to prepare the nutrient medium for a pure culture of a strain of protein microorganisms-producers and its cultivation, it is necessary to add 30% of the filtrate to the grains, while the proportion of crude protein in the protein-containing additive reaches more than 40%. The resulting protein-enriched product has a balanced amino acid composition, which is of interest as a protein-containing feed additive and its fine particle size distribution allows its use for feeding farm animals.
