Sirvi Autor "Kusnere, Z." järgi
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Kirje Herbicide-based selection of mutants for improved single cell protein synthesis: application and procedures(Estonian University of Life Sciences, 2024) Raita, S.; Berzina, I.; Kusnere, Z.; Kalnins, M.; Kuzmika, I.; Spalvins, K.Enhancement of industrially important microbial strains using random mutagenesis is widely used. Screening of potential mutants accelerates the selection of mutants with desired properties such as improved synthesis of lipids, carotenoids, enzymes, or increased tolerance to unfavourable conditions. However, random mutagenesis has not been used to improve protein biosynthesis in microorganisms, and a method for screening these mutants has not yet been developed. The present work reviews the new concept of using herbicides as tools for selecting mutant microorganisms with improved protein biosynthesis. Several pure herbicide substances are amino acid inhibitors whose specific action can be used as a selective pressure for screening protein-rich mutants. The article summarises information about thirteen amino acid inhibitors that inhibit microorganisms and provides data on applicable doses and specifics of use. The article contains mutagenesis protocols and mutant selection strategies, supplemented by theoretical considerations for practical application.Kirje Multi-criteria decision analysis of wood waste ash and glass foam: toward sustainable material selection for biomethanation(Estonian University of Life Sciences, 2024) Kusnere, Z.; Lauka, D.; Spalvins, K.The study examines the potential applications of wood waste ash and waste glass, byproducts of various industrial processes, which have conventional applications such as composting and soil improvement. A new development, vulcanised wood ash material, is studied analysed, drawing parallels between its industrial production process and that of clay pellets. Vulcanised wood ash material and glass foam, which are characterised by advantageous chemical and physical properties, are proving to be versatile resources for various technical applications. Employing a systematic decision-making approach, the study utilises multi-criteria decision analysis and the Technique for Order of Preference by Similarity to Ideal Solution method to evaluate materials for biotrickling filter reactors in ex-situ biomethanation. The comparative analysis includes ash filter material, glass foam, and other industry alternatives, emphasising environmental impacts. The findings reveal expanded clay pellets as the most suitable carrier material, closely followed by polyurethane foam, while glass foam demonstrates remarkable performance despite ranking third. The innovative qualities of glass foam, such as high porosity and thermal insulation, position it as a viable option for biotrickling filter reactors, promoting sustainable practices and circular economy principles. However, further development is required to optimise vulcanised wood ash for biomethanation, potentially enhancing its efficiency through pH adjustment and porosity optimisation.Kirje Packing materials for biotrickling filters used in biogas upgrading – biomethanation(2021) Kusnere, Z.; Spalvins, K.; Blumberga, D.; Veidenbergs, I.One of the promising methods of biogas upgrading is biological methanation (biomethanation). During biomethanation process hydrogenotrophic microorganisms use carbon dioxide from biogas and added hydrogen to generate biomethane. Application of biotrickling filter reactors is one of the prospective biotechnologies for methanation where hydrogenotrophic methanogens are immobilized over a material that is used in reactor. Packing materials for biomethanation are critical in terms of hydrogenotrophic methanogens immobilization on the surface of packing material. It acts as support for biofilm growth. Therefore, characteristics of filter material are important parameters that influence the growth of microorganisms and methane production. Factors, such as optimal specific surface area and porosity are important to sustain growth and activity of microorganisms. Optimal particle size and capability to mechanically resist compaction ensures avoiding high pressure drop. Optimal particle size also ensures uniform gas flow as gases distribute through the packing material. This review paper summarizes and compare the characteristics of different packing materials important for biomethanation through ex-situ biotrickling filter reactor systems.