Sirvi Autor "Kovaleva, E.G." järgi
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Kirje Comparative study of extraction of soy molasses isoflavones and in vivo bioconversion of daidzein into S-equol in rats models(2021) Slesarev, G.P.; Glukhareva, T.V.; Duru, K.C.; Shevyrin, V.A.; Lyubyakina, P.N.; Kovaleva, E.G.We compared different extraction methods for isolation of isoflavones from soybean molasses. Since conventional extraction methods are time-consuming, expensive and unsustainable, we have compared them with the NADES extraction method, which does not have these disadvantages. Fermentation-assisted technique and extraction with Natural Deep Eutectic Solvents (NADES) were compared to the conventional extraction methods. Based on the results obtained, we selected the optimal technique for isoflavones isolation. Isoflavones were identified by gas chromatography equipped with mass spectrometer (GC-MS), whereas their quantities were determined using high performance liquid chromatography (HPLC). In vivo metabolism of daidzein to S-equol was performed in rats with quantification of a yield of S-equol as a result of daidzein in vivo conversion in rats’ intestines.Kirje Effect of different sugar sources on P. rhodozyma Y1654 growth and astaxanthin production(2020) Kanwugu, O.N.; Shatunova, S.A.; Glukhareva, T.V.; Kovaleva, E.G.Phaffia rhodozyma (also known as Xanthophyllomyces dendrorhous) is one of the most promising natural sources of commercial astaxanthin. It has high growth rates, easy cultivation conditions and able to utilize different carbon substrates. This provides an opportunity to further lower production cost by using industrial waste such as molasses. This research therefore evaluates the growth dynamic and astaxanthin production of P. rhodozyma Y1654 growing on soy and sugar beet molasses-based media. Liquid growth media based on soy molasses (SM), sugar beet molasses (SBM) and glucose (control) as main sugar source with peptone and yeast extract supplementation were inoculated with 48 h old seed culture (grown in standard glucose media: 2.0% glucose, 1.0% peptone, 0.2% yeast extract) and incubated at 20 °C with stirring speed of 180 rpm for 7 days. Samples were taken daily throughout the study period to assess; cell count, dry cell weight (DCW) and amount of astaxanthin. Soy molasses-based media resulted in the highest biomass yield (7.7 g L -1 ) followed by SBM (5.8 g L -1 ). Generally, more than 90% of initial fermentable sugar was consumed at the end of the study. However, about 40% of total sugar in SM was unassimilable by P. rhodozyma Y1654. The highest astaxanthin yield was observed in the control media (77 µg g -1 of DCW). Cultivation of P. rhodozyma Y1654 in SBM resulted in as much as twice (32.8 µg g -1 of DCW) the astaxanthin yield of SM (12.4 µg g -1 DCW). Molasses-based media are good for growth of P. rhodozyma Y1654 but for astaxanthin production, they need further optimization.Kirje Production and analysis of non-traditional beer supplemented with sea buckthorn(2017) Adadi, P.; Kovaleva, E.G.; Glukhareva, T.V.; Shatunova, S.A.; Petrov, A.S.In recent years, there has been a growing demand for non-traditional beer (craft) with different flavours hence the main aim of this research is to produce beer with sea buckthorns (SBT). Brewing software BeerSmith was used to design the Kölch beer. After one month of primary fermentation, SBT were sanitised and crashed before adding into the green beer. Carbonation was done using keg with 1.8 bar of carbon dioxide. Physicochemical properties, microbial load and sensory evaluation of Kölch fruit beer (KFB) were determined. All the physiochemical parameters measured were significantly (P < 0.05) influenced by the fermentation time. The pH dropped from 5.8 ± 0.1 to 3.9 ± 0.1 toward the end of the fermentation. There was no microbial growth when KFB was inoculated in the media. °Brix likewise decreases from 13.3 ± 0.43 to 3.86 ± 0.25. There was a change in the colour of the wort throughout the fermentation from 11.2 ± 0.44 to 32.5 ± 0.56 EBC. A decrease from 1.48 ± 0.02 to 0.86 ± 0.02 mg maltose per 100 ml in the reducing sugar was observed during the entire period of fermentation. A total of 32 volatile compounds were identified. All assessed sensory variables of KFB were significantly different (P < 0.05) and preferred by the panellists, however, foaminess and clarity of KFB should have to be improved. KFB showed higher DPPH radical scavenging activity as compared to other types of beer examined due to biologically active substances contributed by SBT.Kirje Production and investigations of antioxidant rich beverage: utilizing Monascus purpureus IHEM LY2014 - 0696 and various malts(2018) Adadi, P.; Kovaleva, E.G.; Glukhareva, T.V.; Barakova, N.V.Antioxidant evokes numerous health benefits to the consumer as well as stabilisation of the beverages flavours. Therefore, this paper provides detailed information on the application of Monascus purpureus IHEM LY2014 - 0696 in combination with vario us malts in brewing antioxidant rich beverage (ARB). Starter culture Angkak was prepared by solid state bioprocessing (SSB). Single infusion method of mashing was used. Physicochemical parameters, volatile compounds, DPPH (2, 2 - diphenyl - 1 - picrylhydrazyl) r adical scavenging activity and fermentability of the wort were evaluated. Distillation procedure based on pycnometry technique was use to quantify the alcohol content (%ABV). Fermentability of the wort was found to be 97.6 ± 0.46% whilst %ABV was equal to 5.42 ± 0.03 . It was observed that ARB showed a strong DPPH radical scavenging activity of 1.00 × 10 – 4 mol × equ (R 2 = 0.91) whereas 3.43 × 10 - 5 mol × equ (R 2 = 0.81) for wort. The strong antioxidant activity (AOA) is thought to be caused by pigments produced by M. purpureus IHEM LY2014 - 0696 and other compounds originated from the malts and hops utilised in brewing ARB. A total of 4 volatile compounds were identified in the present study. Incidence of microbial load ranged from 2.14 ± 0.04 × 10 7 and 0.8 ± 0.1 × 10 5 for M. purpureus IHEM LY2014 - 0696 and bacterial respectively was observed in the ARB. This study contradicts some previous ones, as the ARB brewed did not take the red pigment produced by the M. purpureus . Panellists generally ex pressed their acceptance for the ARB as they assessed it as a new product, moreover, taking account its health benefits.