Sirvi Autor "Ibrahim, M." järgi
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Kirje Biosynthesis of glycosidase inhibitors on wheat bread wastes hydrolysate medium by Streptomyces sp. 170(2019) Sharova, N.; Ibrahim, M.; Prensiva, A.; Vybornova, T.; Barakova, N.; Manzhieva, B.The aim of the present study is to investigate the potential effect of bread hydrolysate as a novel nutrient medium for cultivating Streptomyces sp. 170 (S.170). Moreover, it evaluates the productivity and inhibitory activity of pancreatic α-amylase inhibitors (PAAI). Bread hydrolysate medium (BHM) and corn starch hydrolysate medium (CHM) prepared with αamylase enzyme concentrations (1.5 and 2.5 units g -1 bread) and (1.5 units g -1 corn starch), respectively were utilized in the study. The Seherde-Blair and modified Akulova methods were applied to evaluate the carbohydrates concentration and the inhibitory activity of the media respectively. Results of bread and corn media were compared to each other. Furthermore, the activity of PAAI synthesized by S.170 was compared to other Streptomyces species. The results showed a significant difference (P < 0.05) between the total simple sugars (glucose + maltose) concentration produced in CHM (27.5%) and BHM prepared with α-amylase 1.5 units (45.1%). Besides, BHM produced by α-amylase 2.5 units demonstrated the maximum total concentration of simple sugars (49.9%). In addition, 48 h of S.170 incubation were quite enough to exhibit the highest inhibitory activity (2,632 IU mL-1 ) in BHM prepared with α-amylase 2.5 units. The analysis demonstrated a non-significant difference in the inhibitory activity of PAAI in CMH (1,300 IU mL-1 ) and BMH with α-amylase 1.5 units (1,111 IU mL-1 ). Also, compared to other Streptomyces species, S.170 conferred highly active PAAI. In conclusion, BHM showed its efficiency to a great extent in the cultivation of S.170 and production of PAAI with a notable high activity.Kirje Enrichment of the low-fat yoghurt with oat β-glucan and EPS-producing Bifidobacterium bifidum improves its quality(2020) Ibrahim, M.; Barakova, N.; Jõudu, Ivi; Estonian University of Life Sciences. Institute of Veterinary Medicine and Animal Sciences. Chair of Food Science and Technology; Estonian University of Life Sciences. ERA Chair for Food (By-) Products Valorisation Technologies of the Estonian University of Life SciencesThe addition of β-glucan or EPS-producing bacteria is mainly used to improve the quality and the acceptability of low-fat yoghurt. The purpose of this study was to investigate the effect of adding β-glucan, EPS-producing Bifidobacterium bifidum, or both on physical properties, fermentation time, and organoleptic criteria of low-fat yoghurt, additionally to the viability of L. bulgaricus and B. bifidum. Two types of low-fat yoghurt (1.5% fat) were prepared, with the addition of standard oat β-glucan by 0.15% or without its addition. Each type of yoghurt mixture was inoculated with two kinds of starters: traditional and probiotic (B. bifidum) culture. The physicochemical properties, the count of viable bacterial starter culture, and the organoleptic evaluation for all yoghurt types were evaluated after storage 24 h at 4 °C. Moreover, the fermentation time was monitored. The incorporation of both β-glucan and EPS in yoghurt resulted in the highest viscosity (13.7 mPa.s) and WHC (55.94%), besides to the lowest syneresis (28.47%). The acidity and pH of the yoghurt samples were significantly affected (p > 0.05) by the β-glucan addition. The yoghurt type Bifidobacterium glucan had the shortest fermentation time (215 min), and the maximum viability of both B. bifidum (7.63 Log CFU g -1 ) and L. bulgaricus (7.50 Log CFU g -1 ). The β-glucan had a pronounced effect on the overall acceptability of yoghurt more than the EPS. In conclusion, enriching the low-fat yoghurt with oat β-glucan and EPS-producing B. bifidum is the highest effective method for improving the yoghurt’s quality and the viability of probiotics.Kirje The potential and limitations for applications of oat proteins in the food industry(Estonian University of Life Sciences, 2022) Ibrahim, M.; Aav, Alice; Jõudu, Ivi; Estonian University of Life sciences. Institute of Veterinary Medicine and Animal Sciences. Chair of Food Science and Technology; Estonian University of Life sciences. Institute of Veterinary Medicine and Animal Sciences. ERA-Chair for Food (By-) Products Valorisation Technologies (VALORTECH)Oat proteins have gained high attractive popularity in the market as future protein alternatives in various food products. The extracted oat protein fractions are characterised by a relatively high protein content and a unique amino acid profile compared to other cereal grains. From another aspect, the oat protein is separated unintentionally during the production of oat flours, oat drinks, and oat flakes which encourages the incorporation of oat proteins in the food industry to valorise the food wastes. Therefore, commercial oat proteins possess poor technofunctionality and water solubility in the usual environmental conditions for most food products; therefore, modification of oat proteins functionalities is highly recommended. Several modification methods, including chemical, physical and enzymatic, have been proposed to improve the techno-functionality of native oat proteins and their biological activities. This review highlights the nutritional value of the oat protein fractions, their techno-functional properties and their food industrial challenging limitations. Additionally, it summarises several prospective methods effective for boosting the functionality of oat protein fractions and broadening their application in a range of food industries (bakery, dairy, meat, and their alternative products) with an overview of their impact on humans, animals, and environmental health.Kirje The potential and limitations for applications of oat proteins in the food industry(Estonian University of Life Sciences, 2022) Ibrahim, M.; Aav, Alice; Jõudu, IviOat proteins have gained high attractive popularity in the market as future protein alternatives in various food products. The extracted oat protein fractions are characterised by a relatively high protein content and a unique amino acid profile compared to other cereal grains. From another aspect, the oat protein is separated unintentionally during the production of oat flours, oat drinks, and oat flakes which encourages the incorporation of oat proteins in the food industry to valorise the food wastes. Therefore, commercial oat proteins possess poor technofunctionality and water solubility in the usual environmental conditions for most food products; therefore, modification of oat proteins functionalities is highly recommended. Several modification methods, including chemical, physical and enzymatic, have been proposed to improve the techno-functionality of native oat proteins and their biological activities. This review highlights the nutritional value of the oat protein fractions, their techno-functional properties and their food industrial challenging limitations. Additionally, it summarises several prospective methods effective for boosting the functionality of oat protein fractions and broadening their application in a range of food industries (bakery, dairy, meat, and their alternative products) with an overview of their impact on humans, animals, and environmental health.
