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Sirvi Autor "Honcharov, Y." järgi

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  • Pisipilt
    Kirje
    Phylogenetic diversity and relationships among sorghum genotypes of breeding collection
    (Estonian University of Life Sciences, 2024) Honcharov, Y.; Yalanskyi, O.; Prysiazhniuk, L.; Dikhtiar, I.; Melnyk, S.; Hryniv, S.; Tahantsova, M.; Holichenko, N.
    Sorghum is gaining prominence as a biofuel crop, currently taking the position as the second-largest source of grain-based ethanol after maize. Estimation of genetic diversity to identify groups with similar genotypes is important for conserving, evaluating and utilizing genetic resources. This study aimed to evaluate the genetic diversity and population structure in different genotypes of sorghum collection for breeding purposes to improve cultivars. There were investigated thirty-one sorghum genotypes of different origin. The genetic diversity of sorghum genotypes was assessed by five SSR markers. To evaluate morpho-agronomical traits, days to flowering, plant high, 1,000 seeds weight and yield were studied. As the result of analysis, four cluster groups were formed based on Roger’s & Tanimoto dissimilarity. These cluster groups included from three to sixteen sorghum genotypes, one genotype K2105 formed the separate cluster. The Shannon index calculated based on SSR markers was 1.99. Two principal components explained approximately 63% of the total variance. The greatest effect of year weather conditions was observed on the trait ‘days to flowering’. The plant height was affected by the genotype of grain sorghum. The yield and 1,000 seed weight were affected by weather conditions, but the impact rate was significantly lower than the effect of days to flowering. The correlation between SSR markers and trait ‘days to flowering’ based on distances matrices was weak, but significant (r = 0.1). Thus, obtained results can be utilized for revealing genetic variation and identifying slightly different genotypes in a sorghum breeding program.
  • Pisipilt
    Kirje
    The selection of maize parent lines within marker assisted selection (MAS) by crtRB1-3'TE marker for Steppe zone of Ukraine
    (Estonian University of Life Sciences, 2023) Prysiazhniuk, L.; Honcharov, Y.; Melnyk, S.; Kliachenko, O.
    Maize has a large genotypic diversity and a broad scale of economically important traits. Therefore, it is extremely important for breeding to obtain hybrids which can ensure high yield even under severe growing conditions, such as in Steppe zone of Ukraine. This study aimed to determine the optimal allele ratio by crtRB1-3'TE marker in parental components of maize modified hybrids. There were investigated four hundred sixteen maize hybrids which are modified hybrids of heterotic model (Iodent × Iodent) × Lancaster germplasm. SCA (specific combining ability) effects for grain yield and grain moisture content were calculated in maize hybrids with different allele combinations of crtRB1 gene. As results, the stable positive SCA effects were calculated for hybrids with allele combination (296 bp + 875 bp) × 543 bp × 296+875 bp during both 2019 and 2020 (1.23 and 0.99 t ha-1 , respectively). The lowest SCA effects for grain moisture content were obtained for modified hybrids with allele combination (543 bp × 543) × 296 + 875 bp both in 2019 and 2020 (-0.54 and -0.36%, respectively). The greatest influence SCA effects for grain yield had the interaction of allele combinations and year weather conditions (39%), the impact the allele combinations was 36%. The year weather conditions had the greatest impact on SCA effects for grain moisture content (44%), the allele combination (36%). Thus, it was determined that SCA effects for studied indicators of heterotic model (Iodent × Iodent) × Lancaster under contrasting weather condition are resulted from both genotypes of hybrid parents and favourable allele presence.
  • Pisipilt
    Kirje
    The use of DNA markers for the evaluation of maize lines and hybrids based on cytoplasmic male sterility
    (2020) Prysiazhniuk, L.; Honcharov, Y.; Chernii, S.; Hryniv, S.; Melnyk, S.
    The use of cytoplasmic male sterility (CMS) is very important for the production of maize hybrids. The new inbred lines and hybrids of maize of Ukrainian breeding were studied. In the field, four pairs of sterile analogue of line RL106S were obtained during four backcrossing (17М, 19М, 23М, 27М and 29М) and maintainer lines of RL106fS (18М, 20М, 24М, 28М and 30М) for S type cytoplasm and RL108C (195С, 201С, 205С, 207С, 209С) і RL108fC (196С, 202С, 206С, 208С, 210С) for C type respectively. For S type, the following combinations were obtained: RL23S×RL106fS, RL107S×RL106fS, RL98S×RL106fS, RL105S×RL106fS, RL113S×RL106fS and for C type: RL109C×RL108fC, RL110C×RL108fC, RL112C×RL108fC, RL114C×RL108fC, RL115C×RL108fC. The obtained hybrid combinations were planted the following year in a control nursery for field trials. According to the results of the field assessment, all the hybrids were sterile. The types of sterility of the studied lines and hybrids were determined using polymerase chain reaction (PCR) with specific primers for C and S types of cytoplasms. The presence of specific amplicons 398 and 799 bp was determined in sterile lines with C and S types of cytoplasm, respectively. Amplicons 398 and 799 bp were identified in simple-cross and simple reconstituted hybrids on a sterile basis, and can be used to determine the type of hybrid and its maternal component at the stages of selection and examination of new hybrids.