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Sirvi Autor "Hartatik, S." järgi

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  • Pisipilt
    Kirje
    Evaluation of salt tolerance in sugarcane mutant clone M4 through the application of a rhizobacterial consortium
    (Estonian University of Life Sciences, 2025) Hartatik, S.; Wildana, F.; Gusti, A.A.H.; Aurela, R.D.; Setiyono; Avivi, S.; Kim, K.M.; Ubaidillah, M.
    Sugarcane is one of the most important commodities in the world, with average global production reaching 1.9 billion tonnes in 2022. However, the availability of productive land does not meet the high production demand. This encourages farmers to utilize suboptimal land to meet global sugar cane needs. The aim of this study was to utilise a rhizobacterial consortium to identify the sugarcane mutant clone M4's salt tolerance, which was compared to the widely used Bululawang variety. The experiment was conducted using a hydroponic system with salt treatment (150 mM NaCl) and varying concentrations of the rhizobacterial consortium (2, 4, and 6 mL). Morphological and physiological parameters were measured to assess the response to salt stress. The findings showed that the M4 clone significantly improved plant height, root length, and total chlorophyll content compared to Bululawang. The application of the rhizobacterial consortium significantly enhanced salt tolerance in both genotypes, with the M4 clone showing a stronger response. Overall, the M4 clone displayed greater potential for cultivation in high-salinity soils, particularly when supported by the application of a rhizobacterial consortium. These findings provide valuable insights for the development of sugarcane varieties with enhanced tolerance to abiotic stress, potentially improving agricultural productivity in suboptimal lands.
  • Pisipilt
    Kirje
    Genetic diversity analysis of Indonesian rice germplasm (Oryza sativa L.) with simple sequence repeat markers
    (Estonian University of Life Sciences, 2022) Puspito, A.N.; Nabilah, S.; Imam Buqori, D.M.A.; Hartatik, S.; Kim, Kyung-Min; Ubaidillah, M.
    The characterization of germplasm provides information on the regional rice genetic diversity and variety kinship classification. This study aimed to provide information on the agromorphological traits and genetic diversity of fifty local rice varieties from Java and Borneo Island in Indonesia. The variability of thirteen agronomic traits showed the differentiation among t he accessions, while the phenotypic traits were grouped into six clusters. The genotyping characterization was conducted using SSR (Simple Sequence Repeats) markers (22 microsatellites), and continued with genetic diversity and Polymorphism Information Content (PIC) analysis. The agro-morphological clustering based on Ward’s Hierarchical constructed six sub-clusters. The PC1 and PC2 had 86.3% of the total percentage. The UPGMA method was used to construct six different groupings, as the correlation between each group and its collecting source was significant. Furthermore, the UPGMA dendrogram clustered the 50 accessions into six main clusters, while the PIC showed a polymorphism value range of 0.41–0.74. RM162 located on chromosome 5, which was considered as the best marker for fifty-one genotypes. At the same time, the lowest PIC value of 0.41 was observed in RM431 located in chromosome 1. This classification can be helpful as a detailed information for plant breeders to characterize and select the germplasm, while conducting backcrosses between rice accessions.
  • Pisipilt
    Kirje
    Response of regulation of resistance genes, reactive oxygen species, and antioxidant enzymes to salicylic acid treatments in drought tolerant rice
    (Estonian University of Life Sciences, 2023) Hartatik, S.; Oktaviani, F.; Mufadilah, M.A.; Avivi, S.; Thamrin, N.; Indrawati, A.; Puspito, A.N.; Kim, K.M.; Ubaidillah, M.
    Drought is one of the most important issues in crop production which may disrupt physiological processes and biochemical metabolism in rice plants, including the emergence of plant resistance gene expression such as OsAB13 and OsLEA, the formation of ROS (Reactive Oxygen Species), namely hydrogen peroxide (H2O2), as well as the emergence of gene expression related to antioxidant enzyme activity such as OsAPX1, OsCATA, Mn-SOD, Cu/Zn-SOD, and APX Cytosolic. Besides the emergence of plant resistance gene expression, ROS, and changes in gene expression related to antioxidant enzymes, rice plants also produce salicylic acid which acts as an endogenous signal to activate plant resistance gene responses and can encourage plant resistance responses such as antioxidant enzyme activity. The treatments in this study included: control, 15% PEG 6000, 15% PEG 6000 + SA 1 mM, and SA 1 mM. The results showed that the interaction between treatments and rice plant varieties significantly affected plant height, root length, total chlorophyll, and H2O2 content. The expression of the OsAPX1, OsCATA, Mn-SOD, and Cu/Zn-SOD genes increased in the 15% PEG 6000 treatment compared to the control. In the 15% PEG 6000 + SA 1 mM treatment, there was an increase in gene expression of APX Cytosolic, Cu/Zn-SOD, and OsCATA compared to the 15% PEG 6000 treatment. The OsLEA is expressed in Siakraya and Sertani 1 as an indicator of resistance to drought stress, and the OsAB13 is expressed in Indragiri, Sertani 1, and Siakraya varieties as an indicator of resistance to drought stress.