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Sirvi Autor "Gaboun, F." järgi

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  • Pisipilt
    Kirje
    Development of an efficient regeneration system for bombarded calli from immature embryos of Moroccan durum wheat varieties
    (2019) Senhaji, C.; Gaboun, F.; Abdelwahd, R.; Udupa, S.M.; Douira, A.; Iraqi, D.
    One of the biggest obstacles limiting genetic transformation of durum wheat is the lack of an efficient regeneration system for bombarded tissues. Our study aims to optimize culture conditions for regenerating bombarded calli from immature embryos of four durum wheat varieties ‘Amria’, ‘Chaoui’, ‘Isly’ and ‘Marouane’, through comparing the effects of phytohormones (IAA, zeatin and their interaction) and nitrogen amount and sources on callus induction and plant regeneration. Both tested induction media induced approximately the same rate of induced calli for all the tested varieties. However, the interaction of the induction and the regeneration media showed a highly significant effect on plantlet regeneration for all tested varieties. After bombardment, IM1/RM2 combination proved to be the favourable medium with up to 200% and 120% plantlets regenerated for ‘Chaoui’ and ‘Isly’ varieties respectively. Encouraging results obtained in this study will help to promote the research in genetic transformation and its improvement.
  • Pisipilt
    Kirje
    Molecular Identification and Characterization of Botrytis spp. from Strawberry in Morocco
    (Estonian University of Life Sciences, 2025) Hammoumi, S.; Bentata, F.; Khalifi, H.; Karim, S.; Maafa, I.; Elwahab, F.; Gaboun, F.; Brhadda, N.; Ziri, R.; Labhilili, M.
    Botrytis cinerea is a highly destructive infection, responsible for gray mold in small fruit crops such as strawberries, it causes pre and post-harvest losses. Identification of this pathogen is the first step to control it. The purpose of the present study was to identify Botrytis species, characterize and assess genetic diversity of gray mold pathogen populations from strawberry in Morocco using molecular markers. Sixty-eight isolates were obtained from infected fruits of 14 different geographic origins in Morocco, DNA was extracted and the isolates were identified using Bc108+/Bc563− and NEP2 sequences variability. To differentiate between groups N and S genotypes of Botrytis spp., two primer pairs were used (BcinN-in-F/R and Mrr1_spez_F/R). Presence of transposable elements (TE) boty, flipper and 9 microsatellites (SSR) were used to examine genetic diversity of the isolates. In our population, forty-seven isolates were identified as B. cinerea. Data indicated that 26% of isolates were attributed to groups S and 31% to N. It also showed that 32% of the isolates possessed flipper genotype, followed by transposa (28%), boty (21%) and vacuma (19%). Analysis of 68 Botrytis spp. isolates by SSR showed a high level of genetic diversity indices among populations of which the isolates clustered into seven genetic groups. Data showed genetic diversity in Botrytis spp. populations from Morocco. Identification of the pathogen and knowledge of its genetic diversity enable optimal and effective disease management.