Sirvi Autor "Brhadda, N." järgi
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Kirje Molecular characterization of new causative agents of root rot of wheat in Morocco(Estonian University of Life Sciences, 2024) Khalifi, H.; Bentata, F.; Bouarda, J.; El Aissami, A.; Niya, I.; Kahama Issa, A.; Maafa, I.; Hammoumi, S.; Karim, S.; Ibriz, M.; Amrani Joutei, K.; Brhadda, N.; Ziri, R.; Labhilili, M.Most of the world's cereal-growing regions are severely constrained by root rots, crown rot and head blight brought on by Fusarium spp. In Morocco, yield losses due to root rots are not negligible and range from 12 to 14%. For this study, wheat root rot was surveyed in wheat fields from 2014 to 2019 in different regions of Morocco. Diseased plants are less vigorous, show progressive rotting of the root system and produce white or discolored heads containing stunted seeds. Therefore, the improvement of national production goes through the study of this disease on a deep level. To do this, 75 samples have been collected for the morphological study, which made it possible to identify the genus Fusarium present in the roots and the crown of the infected plant, and the molecular study made it possible to characterize the Fusarium species that are present in Moroccan wheat fields. Molecular identification revealed the presence of five Fusarium species, namely: Fusarium culmorum, which is noted as the dominant species in Morocco with a relative frequency of 21%, F. graminearum, F. equiseti, F. avenaccum and finally F. sambucinum, which represented a high rate in the Gharb region.Kirje Molecular Identification and Characterization of Botrytis spp. from Strawberry in Morocco(Estonian University of Life Sciences, 2025) Hammoumi, S.; Bentata, F.; Khalifi, H.; Karim, S.; Maafa, I.; Elwahab, F.; Gaboun, F.; Brhadda, N.; Ziri, R.; Labhilili, M.Botrytis cinerea is a highly destructive infection, responsible for gray mold in small fruit crops such as strawberries, it causes pre and post-harvest losses. Identification of this pathogen is the first step to control it. The purpose of the present study was to identify Botrytis species, characterize and assess genetic diversity of gray mold pathogen populations from strawberry in Morocco using molecular markers. Sixty-eight isolates were obtained from infected fruits of 14 different geographic origins in Morocco, DNA was extracted and the isolates were identified using Bc108+/Bc563− and NEP2 sequences variability. To differentiate between groups N and S genotypes of Botrytis spp., two primer pairs were used (BcinN-in-F/R and Mrr1_spez_F/R). Presence of transposable elements (TE) boty, flipper and 9 microsatellites (SSR) were used to examine genetic diversity of the isolates. In our population, forty-seven isolates were identified as B. cinerea. Data indicated that 26% of isolates were attributed to groups S and 31% to N. It also showed that 32% of the isolates possessed flipper genotype, followed by transposa (28%), boty (21%) and vacuma (19%). Analysis of 68 Botrytis spp. isolates by SSR showed a high level of genetic diversity indices among populations of which the isolates clustered into seven genetic groups. Data showed genetic diversity in Botrytis spp. populations from Morocco. Identification of the pathogen and knowledge of its genetic diversity enable optimal and effective disease management.Kirje Review of agronomic and genetic diversity of Moroccan rice varieties, and their resistance to blast disease (Pyricularia oryzae)(Estonian University of Life Sciences, 2023) Elwahab, F.; Sedki, M.; Brhadda, N.; Ziri, R.The study on agronomic and genetic characteristics of rice has given us scope to select varieties with desirable characteristics to mitigate various constraints. Rice (Oryza sativa) is the staple food for half of the world's population. However, its production is hampered by a variety of biological constraints. The Blast disease (Pyricularia oryzae) is an important rice disease, and one of the most effective control methods is to use resistant varieties. Study areas in Morocco include the Gharb plains. For all methods, cultural practises like soil levelling seem to be important, but biological control is not widely adopted due to cost, efficacy, and climatic conditions. The bibliographic synthesis was carried out in this context with the main goals of contributing to a better understanding of rice cultivation in Morocco; to identify and characterise the structure of the rice blast pathogen (Pyricularia oryzae), which will allow us to characterise the effects of rice blast; and to research on the Gharb rice field, which resulted in resistant varieties, which will potentially allow producers to have resistant varieties to overcome the diseases. The introduction and development of new rice varieties with high agronomic and socioeconomic value; the selection of lines with high yield, good grain quality, and precocity that are adapted to Moroccan conditions; as well as the development of new lines from Moroccan rice, are among the specific goals.
