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Veterinaarmeditsiini ja loomakasvatuse instituut

Selle valdkonna püsiv URIhttp://hdl.handle.net/10492/2469

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Sirvi Veterinaarmeditsiini ja loomakasvatuse instituut Autor "Ahlstedt, Victoria" järgi

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    The immunolocalization of the Na+ /glucose cotransporter 1 in ostriches’ intestinal epithelium
    (Eesti Maaülikool, 2024) Ahlstedt, Victoria; Hussar, Piret (advisor); Dūrītis, Ilmārs (advisor)
    Glucose transporters are necessary for the absorption of monosaccharides from the small intestinal lumen into the body, thus knowledge about them is crucial for understanding the digestive capacity and nutritional requirements of different animals. Up to now, there has been a gap in the knowledge of the Na+ /glucose cotransporter 1 (SGLT1) – a transporter essential for glucose uptake - in the ostriches’ small intestine. The aim of this cross-sectional study was to immunolocalize SGLT1 in the duodenum and terminal zone of ileum in 1-, 14- , and 28-day-old ostriches to obtain new information about the small intestine’s ability to transport glucose at a young age. Intestinal specimens were collected from 15 female ostriches (Struthio camelus var. domesticus) raised in a Latvian ostrich farm (Ozolini AB), with five ostriches per age group. The samples were fixed with 10% formalin, embedded into paraffin, and sliced into 7 µm thick cuts according to standardized histological procedures. The samples were routinely stained with hematoxylin and eosin, and thereafter, stained immunohistochemically (IHC) using Rabbit anti-SGLT1 (Abcam, UK) as primary antibody and the corresponding immunohistochemistry kit (Abcam, UK) according to the manufacturer’s guidelines. Photos of the samples were taken with a camera (AxioCam, HRc, Germany) and saved on the computer for analysing. The results showed that the expression of SGLT1 got progressively stronger with age, indicating that glucose transport, and thus absorption, is still developing in the small intestine during the first month of life. The findings provides valuable information about ostriches’ nutritional requirements and is suggestive of ostriches not being able to properly utilize carbohydrates from feed at young age.