Optimisation of biologically active compounds ultrasound assisted extraction from potatoes using response surface methodology
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Potato ( Solanum tuberosum L.) is source of p henolic compounds and from plant matrixes can be extracted by several methods. In recent years ultrasound assisted extraction has become more popular due to its efficiency for recovery of phenolic compounds and antioxidants and response surface methodology is an effective tool for optimisation of extraction procedure by evaluating different variables and their interaction. The aim of the current research was to optimize ultrasound assisted extraction of biologically active compounds from potatoes by respons e surface methodology. For experiment purple - flesh potato variety ‘Blue Congo’ was selected. Control sample was extracted by stirring for 1 hour. Box - Behnken design was used for optimization of extraction conditions from fresh potatoes and as variables wer e selected: ethanol concentration (% v/v), hydrochloric acid concentration (molarity) and time (min). For extracts as responses total phenolic, total flavonoid, total anthocyanin content and antioxidant activity (DPPH, ABTS+ scavenging activity) were deter mined using a spectrophotometric methods. Significant models were obtained for antocyanins, total phenols and DPPH radical scavenging activity. Optimisation of extraction showed that for maximising all responses optimal HCl concentration is 2.5M, ethanol c oncentration 79.4% and extraction time 60 minutes, resulting in following responses: 57.41 mg 100 g - 1 of anthocyanins, 238.52 mg 100 g - 1 of TPC, 24.58 mM TE 100 g - 1 of DPPH scavenging activity and 12.99 mM TE 100 g - 1 of ABTS scavenging activity. Convention al extraction method showed significantly lower results. It could be concluded that ultrasound assisted extraction is effective method for recovery of phenolic compounds and solvents and extraction time is significant parameter influencing efficiency.