Establishment of in vitro propagation protocol of Gerbera jamesonii Bolus ex Hook f.: explant and media selection to plantlet acclimatization
Prama Yufdy, Muhammad
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Gerbera (Gerbera jamesonii Bolus ex Hook f.) is an important ornamental plant commodity with high economical value in Indonesia; however, development of the plant in larger scale commercially is constrained by the availability of qualified-planting materials. Conventional propagation is clearly not suitable to overcome the problem and therefore in vitro propagation protocol is importantly addressed. In vitro propagation protocol of G. jamesonii was successfully established in the study. Different explants (shoot tips, young leaves, petioles and petals) were selected and cultured on Murashige and Skoog (MS) basalt medium containing different plant growth regulators. Shoot tips and half-strength MS medium containing 1.5 mg l-1 thidiazuron (TDZ) and 0.25 mg l-1 N6-benzylaminopurine (BAP) were suitable explant and initiation medium for shoot formation with 75% explant regeneration and 5.5 shoots produced per explant. Shoots derived from selection stage were proliferated on half-strength MS medium supplemented with 0.25 mg l-1 BAP and resulting higher shoot regeneration up to 7.5 shoots produced per shoot with 22.8 leaves and 0.83 cm leaf length. Multiplication of shoots on the half-strength MS medium fortified by 0.25 mg l-1 BAP increased gradually from the first subculture till the fifth subculture with 9.1 shoots produced per shoot subcultured and reduced thereafter. Shoots were then rooted on half-strength MS medium augmented with 0.5 mg l-1 α-naphthalene acetic acid (NAA) and 1.5 g l-1 activated charcoal (AC) and produced 2.1 roots per shoot with 2.52 cm length of roots. Well-rooted shoots were acclimatized in a mixture of burned-rice husk and organic manure (1:1, v/v) with 95% survival rate and 114 plantlets grew well under this treatment.